@ARTICLE{Grzesiak_Jakub_Microbial_2009, author={Grzesiak, Jakub and Żmuda−Baranowska, Magdalena and Borsuk, Piotr and Zdanowski, Marek}, volume={vol. 30}, number={No 1}, journal={Polish Polar Research}, pages={37-47}, howpublished={online}, year={2009}, publisher={Polish Academy of Sciences}, publisher={Committee on Polar Research}, abstract={Since 1978 the retreat of Ecology Glacier in the vicinity of Henryk Arctowski Station has opened new ice-free areas for colonization by terrestrial organisms initiated by pioneer microbes. Samples were collected from the soil surface, at 0, 5 and 20 cm below surface close to glacier front, then stored at below -20°C . Total bacterial count (TC), estimated by epifluorescence microscopy, reached high values, of 1010 g-1 dry wt. Healthy looking bacterial cells of mean volume 0.0209 µm3 at 0 cm to 0.0292 µm3 at 20 cm made up from 7% at 0 cm , to 30% at 20 cm of total bacterial population. The number of colony forming units (CFU) accounted for only 0.02% of TC. Taxonomically they belonged to the a, b, g subdivisions of the proteobacteria and to the Cytophaga-Flavobacterium-Bacteroides (CFB) group. Morphophysiologically CFU bacteria were diverse, from Gram variable short coccal forms to very long rods or filaments. Randomly selected CFU colonies were characterized by low sugar assimilation and high esterase/lipase activity. Spore forming bacteria – absent from 0 and 5 cm , formed a small fraction of 175 cells g-1 dry wt at the 20 cm depth. Filamentous fungi were relatively abundant and represented mainly by oligotrophs.}, type={Article}, title={Microbial community at the front of Ecology Glacier ( King George Island , Antarctica ): Initial observations}, URL={http://www.journals.pan.pl/Content/110539/PDF-MASTER/PPR30-037.pdf}, keywords={Antarctica, glacial retreat, ice-free areas, microbial communities}, }