@ARTICLE{Zhang_T._Estrogenic_2019, author={Zhang, T. and Chi, X.X.}, volume={vol.22}, number={No 1}, journal={Polish Journal of Veterinary Sciences}, pages={83–90}, howpublished={online}, year={2019}, publisher={Polish Academy of Sciences Committee of Veterinary Sciences}, publisher={University of Warmia and Mazury in Olsztyn}, abstract={The purpose of the study was to study the activity of the phytoestrogen genistein (GEN) act- ing on FSHR and LHR in rat ovaries with polycystic ovary syndrome (PCOS). Sixty rats were di- vided into six groups. Rats in the dose group received genistein at a concentration of either 5 (low genistein dose group, L-gen), 10 (middle genistein dose group, M-Gen) or 20 (high genistein dose group, H-Gen) mg per kg of body weight per day. Estrogen group (EG, received 0.5 mg/kg Dieth- ylstilbestrol). Concentration of sex hormones in serum was quantified by enzyme-linked immuno- sorbent assay (ELISA). Expressions of follicle-stimulating hormone receptor (FSHR) and lutein- izing hormone receptor (LHR) protein were determined by immunohistochemistry. Treatment with genistein resulted in a strong stimulation of the concentration of sex hormone in serum. The concentration of progesterone and FSH was significantly higher in H-Gen when compared to the PCOS model control group (MG) (P < 0.01). In contrast, the concentration of testosterone, LH and the ratio of LH/FSH decreased in GEN treatment groups compared to MG, the effect was statistically significant, tested by the ANOVA test (p<0.01). For hormone receptor activity, treat- ment with genistein resulted in an improvement of ovarian function with LHR protein expression being enhanced and FSHR protein expression being suppressed. Our results demonstrate that Genistein played a significant role in regulating FSH and LH receptor expression to improve perimenopausal ovarian and uterine function.}, type={Article}, title={Estrogenic properties of genistein acting on FSHR and LHR in rats with PCOS}, URL={http://www.journals.pan.pl/Content/110875/PDF/12.pdf}, doi={10.24425/pjvs.2019.127075}, keywords={genistein, polycystic ovary syndrome, follicle-stimulating hormone receptor, luteinizing hormone receptor, female rat}, }