@ARTICLE{Chmielewska-Krzesińska_M._Stability_2019,
 author={Chmielewska-Krzesińska, M. and Jakimiuk, A. and Wąsowicz, K.},
 volume={vol.22},
 number={No 3},
 journal={Polish Journal of Veterinary Sciences},
 pages={523-529},
 howpublished={online},
 year={2019},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={αB-crystallin is a member of a small family of thermal shock proteins that protects cells from stress. Because of lack of its expression in peripheral blood leukocytes, it was proposed as a molecular marker of circulating tumor cells in canine mammary gland tumors. The aim of the present study was to determine if αB-crystallin shows stability of expression, what is the requirement for this type of marker. It was also assessed whether there is co-expression of αB-crystallin with the basal marker, cytokeratin 17. For this purpose, samples of various types of canine mammary gland tumors of epithelial origin, were selected. Using RT-qPCR, we have found αB-crystallin and cytokeratin 17 co-expression in benign and malignant canine mammary gland tumors. It has been demonstrated that the expression of αB-crystallin in tested neoplastic samples is not stable in comparison to the control group. Furthermore αB-crystallin overor down- expression was associated witch the same cytokeratin 17 pattern. αB-crystallin can be a marker of circulating tumor cells in the bloodstream, but for cancers in which basal marker expression occurs and thus not universal for all cancers originating from the mammary gland tissue.},
 type={Article},
 title={Stability of αB-crystallin gene expression in canine mammary gland neoplasms. Should it be considered as circulating tumor cell genetic marker?},
 URL={http://www.journals.pan.pl/Content/113456/PDF/11.pdf},
 doi={10.24425/pjvs.2019.129960},
 keywords={αB-crystallin (CRYAB), canine mammary gland tumor, circulating tumor cells (CTC), cytokeratin 17 (CK17)},
}