TY - JOUR N2 - The reliable and rapid diagnosis of infectious animal diseases presents an exceptionally im- portant aspect when considering their control and prevention. The paper describes the compara- tive evaluation of two rapid isothermal amplification methods for diagnosis of African swine fever (ASF). The robustness of loop-mediated isothermal amplification (LAMP) and the cross-priming amplification (CPA) were compared using samples obtained from ASF confirmed animals. Both assays were evaluated in order to define their diagnostic capabilities in terms of ASF diagnosis and reproducibility of the results. Investigations showed no cross-reactivity for other pig patho- gens and no significant differences in the specificity of both assays. The sensitivity of LAMP reached 90%, while that of CPA was 70%. In conclusion, both methods are suitable for imple- mentation in preliminary ASF diagnosis but further improvements are required to enhance their diagnostic sensitivity. L1 - http://www.journals.pan.pl/Content/109589/PDF/24.pdf L2 - http://www.journals.pan.pl/Content/109589 PY - 2018 IS - No 4 EP - 827–830 DO - 10.24425/pjvs.2018.125597 KW - African swine fever KW - loop-mediated isothermal amplification KW - cross-priming amplification KW - comparison KW - diagnostic evaluation A1 - Woźniakowski, G. A1 - Frączyk, M. A1 - Mazur, N. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 21 DA - 2018.12.20 T1 - Comparison of loop-mediated isothermal amplification (LAMP) and cross-priming amplification (CPA) for detection of African swine fever virus SP - 827–830 UR - http://www.journals.pan.pl/dlibra/publication/edition/109589 T2 - Polish Journal of Veterinary Sciences ER -