TY - JOUR N2 - Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adju- vanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response. L1 - http://www.journals.pan.pl/Content/110886/PDF/01.pdf L2 - http://www.journals.pan.pl/Content/110886 PY - 2019 IS - No 2 EP - 195–201 DO - 10.24425/pjvs.2019.127086 KW - adjuvant KW - CpG DNA KW - Echinococcus granulosus KW - TBK1 KW - type I interferons A1 - Wu, J. A1 - Chen, Q. A1 - Xin, T. A1 - Sun, Y. A1 - Jia, H. A1 - Hou, S.H. A1 - Guo, X.Y. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol.22 DA - 2019.07.01 T1 - pUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice SP - 195–201 UR - http://www.journals.pan.pl/dlibra/publication/edition/110886 T2 - Polish Journal of Veterinary Sciences ER -