TY - JOUR N2 - Rare and endemic plant species represent important components of plant biodiversity which require protection to ensure their sustainable conservation. Cerastium banaticum (Rochel) Heuff. is such an endemic and rare species from Romania, for which the genetic variability of two natural populations was studied by SSR markers. Shannon’s information index revealed low levels of genetic diversity in both populations (I = 0.296). As the first attempt in a conservation program a reproducible micropropagation protocol was established starting from seeds, followed by multiplication, rooting, and ex vitro acclimatization. Among the various plant growth regulators tested the highest multiplication coefficient was achieved on a culture medium with 0.5 mg L-1 6-furfurylaminopurine (K) and 1 mg L-1 α-naphthaleneacetic acid (NAA). On this PGRs concentration a number of 26.6 shoots/individual explant with a mean length of 7.9 cm for new generated shoots was registered. The highest number of roots/individual initiated shoot was 2.6 and it was recorded on a culture medium with 0.5 mg L-1 2-isopentyl-adenine (2iP) and 0.1 mg L-1 NAA. The outdoor acclimatization was successfully performed in a specially designed rocky area in the ‘Alexandru Borza’ Botanical Garden, Cluj-Napoca (Romania). L1 - http://www.journals.pan.pl/Content/111454/PDF/ABC%20Bot.%201-19%205-V.Cristea%20i%20in.pdf L2 - http://www.journals.pan.pl/Content/111454 PY - 2019 IS - No 1 EP - 62 DO - 10.24425/abcsb.2019.127737 KW - acclimatization KW - biodiversity conservation KW - plant growth regulators KW - SSR markers A1 - Cristea, Victoria A1 - Besenyei, Enikő A1 - Jarda, Lilianna A1 - Farkas, Anca A1 - Marcu, Delia A1 - Clapa, Doina A1 - Halmagyi, Adela A1 - Butiuc-Keul, Anca PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 61 DA - 2019.05.15 T1 - In Situ Genetic Variability and Micropropagation of Cerastium banaticum (Rochel) Heuff. (Caryophyllaceae)– a Rare and Endemic Species from Romania SP - 53 UR - http://www.journals.pan.pl/dlibra/publication/edition/111454 T2 - Acta Biologica Cracoviensia s. Botanica ER -