The article presents the process of functional changes in the area of the Metropolis of Poznań, i.e. 22 communes belonging to the Metropolia Poznań Association. The process was examined on the basis of changes in the employment structure and spatially different tendencies and segregation in the scope of dominant functions.
The measurement of frequency characteristics, like magnitude and phase, related to a specific transfer function of DC–DC converters, can be a difficult task – especially when the measured signal approaches the boundary of a small-signal model validity (i:e. 1/3 of the switching frequency fS). It is hard to find a paper where authors mention the measurement techniques they use to draw frequency characteristics. Meanwhile the presence of noise in the output signal does not enable to directly measure the gain and the phase shift between the input and output signals. In such situations additional analysis is required in order to achieve a reliable result. This paper contains a description of a few methods that can be used to analyse measured signals in order to determine the gain and the phase shift of a specific transfer function. They enable to verify mathematical models in a wide range of frequencies (up to 1/3 fS). The methods use signals measured in the time domain and can be implemented in mathematical software such as Matlab or Scilab.
Animals as a source of organs and tissues for xenotransplantation could become a backup solution for the growing shortage of human donors. The presence of human xenoreactive anti- bodies directed against Galα1,3Gal antigens on the cell surface of a pig donor triggers the activa- tion of the complement leading to a hyperacute reaction. The development of genetic engineer- ing techniques has enabled the modification of genomes by knocking in and/or knocking out genes. In this paper, we report the generation of modified pigs with ZFN mediated disruption of the GGTA1 gene encoding the enzyme responsible for synthesis of Galα1,3Gal antigens. ZFN plasmids designed to target the exon 9 region of the pig GGTA1 gene encoding the catalytic domain were injected into the pronuclei of fertilized egg cells. Among 107 piglets of the F0 gene- ration analyzed, one female with 9-nt deletion in exon 9 of the GGTA1 gene was found. 13 of 33 piglets of the F1 generation represented the +/- GGTA1 genotype and 2 of 13 F2 piglets repre- sented the -/- GGTA1 genotype. No changes in the animals’ behavior, phenotype or karyotype were observed. Analysis confirmed heredity of the trait in all animals. A complex functional analysis of the modified animals, including flow cytometry, human serum cytotoxicity test and immunohistochemical detection, was performed to estimate the phenotype effect of genetic modification and this indicated an efficient GGTA1 knock-out in modified pigs.