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Abstract

This study focused on the fatigue and corrosion fatigue of maraging steel 18Ni (250). The 18Ni (250) samples were tested for axial fatigue in air and 3.5% NaCl solution. The effects of loading frequency and stress ratio on the fatigue strength of 18Ni (250) were studied. In air, the loading frequency was 10 Hz, and the stress ratio was 0.5. However, three loading methods were used in the 3.5% NaCl solution: (i) the loading frequency of 1 Hz and stress ratio of 0.5; (ii) the loading frequency of 1 Hz and stress ratio 0.1, and (iii) the loading frequency 5 Hz and stress ratio 0.5. The corrosion fatigue strength of samples in the 3.5% NaCl solution was 63.3% lower than that of the samples in air. The fractures in the samples were observed after the test.

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Authors and Affiliations

Zhu Yongmei
Chen Junjie
Tang Wenxian
Cui Weicheng
Wang Xiaorong
Wang Fang
Yin Baoji
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Abstract

Phytoplankton samples were collected at 74 stations in the European Arctic seas, 28 species of the armoured din oft age Hates being found. Thirteen dinoflagellates are illustrated by original drawings. The data on synonyms, size or size variation, localities and environmental factors (temperature and salinity) at the surface are given.

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Authors and Affiliations

Yuri B. Okolodkov
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Abstract

Searching for new refrigerants is one of the most significant scientific problems in refrigeration. There are ecological refrigerants commonly known: H2O and CO2. H2O and CO2 known as natural refrigerants, but they have problems:a high freezing point of H2O and a low triple point of CO2. These problems can be solved by the application of a hybrid sorption-compression refrigeration cycle. The cycle combines the application possibility of H2O in the high temperature sorption stage and the low temperature application of CO2 in the compression stage. This solution gives significant energy savings in comparison with the two-stage compressor cycle and with the one-stage transcritical CO2 cycle. Besides, the sorption cycle may be powered by low temperature waste heat or renewable heat. This is an original idea of the authors. In the paper an analysis of the possible extension of this solution for high capacity industrial refrigeration is presented. The estimated energy savings as well as TEWI (Total Equivalent Warming Impact) index for ecological gains are calculated.

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Authors and Affiliations

Piotr Cyklis
Karina Janisz
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Abstract

One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.

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Authors and Affiliations

Maciej Pilarek
Michał Wojasiński
Klaudia Godlewska
Aleksandra Kuźmińska
Katarzyna Dąbkowska

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