Inoculation of tobacco cv. Xanthi nc or bean plants with the mixtures of benzothiadiazole (Bion) and tobacco mosaic tobamovirus (TMV) or alfalfa mosaic virus (AIMV), respectively did not show any inhibition of the number and size of the local lesions. Protective treatment of plants with Bion caused a significant decrease in disease incidence. In the case of tobacco cv. Xanthi nc and TMV or bean plants and AIMV that protective effect increased day by day and 6-7 days after treatment the production of local lesions was inhibited almost completely. Bean plants treated with Bion demonstrated resistance ranging between 60-90% also in nontreatcd parts. Bean and tomato plants pretreated with O.Ol% Bion were effectively (in 60-70%) protected against systemic infection by tomato black ring ncpovirus (TBRV).

In performed experiments, insoluble polyvinylpolypyrrolidone, PVPP as an additive to the extraction buffer was used for isolation of total nucleic acids from hop plants and grapevine in order to obtain templates useful for detection ofHLVd and HSVd by means ofRT-PCR. Addition of2% of PVPP to the original GTC buffer (Chomczynski and Sacchi, 1987) appeared to be the most favorable. Due to PVPP addition, the protocol of extraction of nucleic acids was simplified by shortening of isolation time and reduction of expenses. However, application of the simplified method for obtaining of templates that guaranteed full repeatability of test results was limited to the spring and early summer season.

A method for obtaining of alkaloid preparations (A, fraction and pure alkaloid perchlorates ofA5 one) from bitter lupin (Lupinus albus L. cv. Bac) extract and evaluation of their usefulness for plant protection are described. The activity of the extract as well as its fraction A, and two major alkaloids of A3 one was tested on pests such as large cabbage white (Pieris brassicae L.) and small cabbage white (Pieris rapae L.) fed with leaves of cabbage treated with these preparations. Fractionation of the extract to obtain A, fraction and alkaloids perch I orates ofA3 one has been sufficient to eliminate impurities that may potentially cause undesirable by-effects in biological tests. Significant changes in various stages of development of insects were observed. Generally, fraction A, of lupin extract caused decrease in mass of fed feed as well as weight of objects observed. Furthermore, numerous visible damages of fed larvae and pupae that caused their inability to generate normal imagines (butterflies) were observed. Perchlorates of lupanine and 13-hydroxylupanine derived from lupin extract A3 fraction have not shown that effect, particularly on P. brassicae. Above supports an evidence that only natural bioconjugates of active substance may perform strong biological activity. In spite of similarity of tested insect species considerable differences between them in reaction to preparations were noticed.

A characteristics of three extracellular metabolites produced by Bacillus coagulans strain that were supposed to be responsible for its fungistatic activity against Trichothecium roseum has been done by TLC, GC-MS and Western blotting methods. It was shown that these compounds of molecular weights as 41, 45 and 65 kDa secreted to the medium by bacteria had glycoprotein properties and did not become inactive when boiled for 20 min., as characteristic for glycoprotein.