Abstrakt
In recent years there have been a growing number of reports on applying viruses in oncological treatment. In the present study, we demonstrated for the first time that animal virus EHV-1 productively replicates in the human adenocarcinoma cell line (A549) without the need for adaptation. Real-time PCR analysis and immunofluorescence assay showed that EHV-1 could infect and causes lysis of human lung cancer cells. According to our results, we can assume that EHV-1 has oncolytic potential.
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Bibliografia
Chodkowski M, Cymerys J, Słońska A, Bańbura M (2017) Oncolytic animal viruses and their applications in anti-cancer therapies. Med Weter 73: 4-9.
Courchesne MJ, White MC, Stanfield BA, Frampton AR (2012) Equine herpesvirus type 1-mediated oncolysis of human glioblastoma multi-forme cells. J Virol 86: 2882-2886.
Cymerys J, Dzieciątkowski T, Słońska A, Bierla J, Tucholska A, Chmielewska A, Golke A, Bańbura MW (2010) Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture. Pol J Vet Sci 13: 701-708.
Cymerys J, Słońska A, Brzezicka J, Tucholska A, Chmielewska A, Rola J, Bańbura MW (2016) Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line. Pol J Vet Sci 19: 777-784.
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Słońska A, Cymerys J, Godlewski MM, Bańbura MW (2016) Application of scanning cytometry and confocal-microscopy-based image analysis for investigation the role of cytoskeletal elements during equine herpesvirus type 1 (EHV-1) infection of primary murine neurons. J Virol Met 237:1-9.
White MC, Frampton AR Jr (2013) The histone deacetylase inhibitor valproic acid enhances equine herpesvirus type 1 (EHV-1)-mediated oncolysis of human glioma cells. Cancer Gene Ther 20: 88-93.
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