Alibhai SM, Duong-Hua M, Sutradhar R, Fleshner NE, Warde P, Cheung AM, Paszat LF (2009) Impact of androgen deprivation therapy on cardiovascular disease and diabetes. J clin Oncol. 27: 3452.
Amaya D, Christoffersen P, Jacobs K, Vasquez A (2015) Does realized skewness predict the cross-section of equity returns? J Fin Eco 118: 135-167.
Anjum NA, Singh N, Singh MK, Sayeed I, Duarte AC, Pereira E, Ahmad I (2014) Single-bilayer graphene oxide sheet impacts and underly-ing potential mechanism assessment in germinating faba bean (Vicia faba L.). Sci Total En 472: 834-841.
Bellavance A, Bonneville-Hébert A, Desrochers A, Fecteau G (2010) Surgical correction of a diaphragmatic hernia in a newborn calf. Canad Vet J 51: 767.
Crane R, Dickinson M, Popescu IC, Scott T(2011) Magnetite and zero-valent iron nanoparticles for the remediation of uranium contaminated environmental water. Water Res 45: 2931-2942.
Doijode V (2019). Umbilical hernia in ruminant calves: A review. Pharma Innovation 8: 164-167.
Ergul Z, Akinci M, Ugurlu C, Kulacoglu H, Yilmaz K (2012) Prophylactic antibiotic use in elective inguinal hernioplasty in a trauma center. Hernia 16: 145-151.
Fazili M, Buchoo B, Bhattacharyya H, Khan I (2013) Uncomplicated (simple) umbilical hernia in crossbred dairy calves: management with or without surgery. Indian J Vet Surg 34: 111-114.
Gonzalez R, Mason E, Duncan T, Wilson R, Ramshaw BJ (2003) Laparoscopic versus open umbilical hernia repair. JSLS 7: 323.
Hermann DM, Kilic E, Kügler S, Isenmann S, Bähr M (2001) Adenovirus-mediated glial cell line-derived neurotrophic factor (GDNF) ex-pression protects against subsequent cortical cold injury in rats. Neurobiol Dis 8: 964-973.
Jaman MM, Mishra P, Rahman M, Alam MM (2018) Clinical and laboratory investigation on the recurrence of the umbilical hernia after herniorrhaphy in bovine calves. J Bangladesh Agri Uni 16: 464-470.
Kool M, Jones DT, Jäger N, Northcott PA, Pugh TJ, Hovestadt V, Piro RM, Esparza LA, Markant SL, Remke M, Milde T (2014) Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition. Cancer Cell 25: 393-405.
Marciniak SJ, Yun CY, Oyadomari S, Novoa I, Zhang Y, Jungreis R, Nagata K, Harding HP, Ron D (2004) CHOP induces death by pro-moting protein synthesis and oxidation in the stressed endoplasmic reticulum. Gene Dev 18(24): 3066-3077.
Muschaweck U (2003) Umbilical and epigastric hernia repair. Surg Clin 83: 1207-1221.
Riley CB, Cruz AM, Bailey JV, Barber SM, Fretz PB (1996) Comparison of herniorrhaphy versus clamping of umbilical hernias in horses: a retrospective study of 93 cases (1982-1994). Canad Vet J 37: 295.
Steenholdt C, Hernandez J (2004) Risk factors for umbilical hernia in Holstein heifers during the first two months after birth. J Am Vet Med Assoc 224: 1487-1490.
Udegbunam JE, Fjelde KK, Evje S, Nygaard G (2015) On the advection-upstream-splitting-method hybrid scheme: a simple transient-flow model for managed-pressure- -drilling and underbalanced-drilling applications. SPE Dril Comp 30: 98-109.
Wilhelm M, Hölzer J, Dobler L, Rauchfuss K, Midasch O, Kraft M, Angerer J, Wiesmüller G (2009) Preliminary observations on perfluori-nated compounds in plasma samples (1977–2004) of young German adults from an area with perfluorooctanoate-contaminated drinking water. Int J Hyg En Health 212: 142-145.

1. Adu-Bobie J, Capecchi B, Serruto D, Rappuoli R, Pizza M (2003) Two years into reverse vaccinology. Vaccine 21: 605-610.
2. Bock RE, De Vos AJ (2001) Immunity following use of Australian tick fever vaccine: a review of the evidence. Aust Vet J 79: 832-839.
3. Callow LL, Dalgliesh RJ, De Vos AJ (1997) Development of effective living vaccines against Bovine Babesiosis – the longest field trial? Int J Parasitol 27: 747-767.
4. Carcy B, Précigout E, Schetters T, Gorenflot A (2006) Genetic basis for GPI-anchor merozoite surface antigen polymorphism of Babesia and resulting antigenic diversity. Vet Parasitol 138: 33-49.
5. Caro-Gomez E, Gazi M, Goez Y, Valbuena G (2014) Discovery of novel cross-protective Rickettsia prowazekii T-cell antigens using a combined reverse vaccinology and in vivo screening approach. Vaccine 32: 4968-4976.
6. Çiloğlu A, Abdullah IN, Yildirim A, Önder Z, Düzlü Ö (2018) Molecular characterization and expression of the apical membrane antigen-1 from in vivo and in vitro isolates of Babesia bigemina Kayseri/Turkey strain. Ankara Univ Vet Fak Derg 65: 239-246.
7. de Castro JJ (1997) Sustainable tick and tickborne disease control in livestock improvement in developing countries. Vet Parasitol 71: 77-97.
8. Debierre-Grockiego F, Schwarz RT (2010) Immunological reactions in response to apicomplexan glycosylphosphatidylinositols. Glycobiology 20: 801-811.
9. Debierre-Grockiego F, Smith TK, Delbecq S, Ducournau C, Lantier L, Schmidt J, Brès V, Dimier-Poisson I, Schwarz RT, Cornillot E (2019) Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cells. Biochimie 167: 135-144.
10. Delbecq S, Hadj-Kaddour K, Randazzo S, Kleuskens J, Schetters T, Gorenflot A, Précigout E (2006) Hydrophobic moeties in recombinant proteins are crucial to generate efficient saponin-based vaccine against Apicomplexan Babesia divergens. Vaccine 24: 613-621.
11. Djokic V, Akoolo L, Parveen N (2018) Babesia microti infection changes host spleen architecture and is cleared by a Th1 immune response. Front Microbiol 9:85
12. Durrani A, Kamal N (2008) Identification of ticks and detection of blood protozoa in Friesian cattle by polymerase chain reaction test and estimation of blood parameters in district Kasur, Pakistan. Trop Anim Health Prod 40: 441-447.
13. Echaide IE, De Echaide ST, Guglielmone AD (1993) Live and soluble antigens for cattle protection to Babesia bigemina. Vet Parasitol 51: 35-40.
14. Esmaeilnejad B, Tavassoli M, Asri-Rezaei S, Dalir-Naghadeh B, Mardani K, Golabi M, Arjmand J, Kazemnia A, Jalilzadeh G (2015) Determination of prevalence and risk factors of infection with Babesia ovis in small ruminants from West Azerbaijan Province, Iran by polymerase chain reaction. J Arthropod Borne Dis 9: 246-252.
15. Ezediuno LO, Onile OS, Oladipo EK, Majolagbe ON, Jimah EM, Senbadejo TY (2021) Designing multi-epitope subunit vaccine for ocular trachoma infection using Chlamydia trachomatis polymorphic membrane proteins G. Inform Med Unlocked 26: 100764.
16. Fisher TG, McElwain TF, Palmer GH (2001) Molecular basis for variable expression of merozoite surface antigen gp45 among American isolates of Babesia bigemina. Infect Immun 69: 3782-3790.
17. Gaafar B, Ali SA, Abd-Elrahman KA, Almofti YA (2019) Immunoinformatics approach for multiepitope vaccine prediction from H, M, F, and N proteins of Peste des Petits ruminants virus. J Immunol Res 2019. 6124030
18. Gomara MJ, Haro I (2007) Synthetic peptides for the immunodiagnosis of human diseases. Curr Med Chem 14: 531-546.
19. Hajissa K, Zakaria R, Suppian R, Mohamed Z (2019) Epitope-based vaccine as a universal vaccination strategy against Toxoplasma gondii infection: A mini-review. J Adv Vet Anim Res 6: 174-182.
20. Jabbar A, Abbas T, Sandhu Z, Saddiqi HA, Qamar MF, Gasser RB (2015) Tick-borne diseases of bovines in Pakistan: major scope for future research and improved control. Parasit Vectors 8:.283
21. Jorgensen WK, De Vos AJ, Dalgliesh RJ (1989) Comparison of immunogenicity and virulence between Babesia bigemina parasites from continuous culture and from a splenectomised calf. Aust Vet J 66: 371-372.
22. Kalita J, Padhi AK, Tripathi T (2020a) Designing a vaccine for fascioliasis using immunogenic 24 kDa mu-class glutathione s-transferase. Infect Genet Evol 83: 104352.
23. Kalita P, Padhi AK, Zhang KY, Tripathi T (2020b) Design of a peptide-based subunit vaccine against novel coronavirus SARS-CoV-2. Microb Pathog 145: 104236.
24. Kar PP, Srivastava A (2018) Immuno-informatics analysis to identify novel vaccine candidates and design of a multi-epitope based vaccine candidate against Theileria parasites. Front Immunol 9: 2213.
25. Karim S, Budachetri K, Mukherjee N, Williams J, Kausar A, Hassan MJ, Adamson S, Dowd SE, Apanskevich D, Arijo A, Sindhu ZU (2017) A study of ticks and tick-borne livestock pathogens in Pakistan. PLOS Negl Trop Dis 11: e0005681.
26. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T ( 2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647-1649.
27. Khatoon N, Ojha R, Mishra A, Prajapati VK (2018) Examination of antigenic proteins of Trypanosoma cruzi to fabricate an epitope-based subunit vaccine by exploiting epitope mapping mechanism. Vaccine 36: 6290-6300.
28. Klafke G, Webster A, Agnol BD, Pradel E, Silva J, de La Canal LH, Becker M, Osório MF, Mansson M, Barreto R, Scheffer R (2017) Multiple resistance to acaricides in field populations of Rhipicephalus microplus from Rio Grande do Sul state, Southern Brazil. Ticks Tick Borne Dis 8: 73-80.
29. Kumar P, Lata S, Shankar UN, Akif M (2021) Immunoinformatics based designing of a multi- epitope chimeric vaccine from multi-domain outer surface antigens of Leptospira. Front Immunol 12: 735373.
30. Lew-Tabor AE, Valle MR (2016) A review of reverse vaccinology approaches for the development of vaccines against ticks and tick borne diseases. Ticks Tick Borne Dis 7: 573-585.
31. List C, Qi W, Maag E, Gottstein B, Müller N, Felger I (2010) Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. PLOS Negl Trop Dis 4: e771.
32. Maiorano AM, Giglioti R, Oliveira MC, Oliveira HN, Cyrillo JN, Mercadante ME, Silva JA (2018) Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil Anim Prod Sci 59: 938-944.
33. Mangold AJ, Vanzini VR, Echaide IE, De Echaide ST, Volpogni MM, Guglielmone AA (1996) Viability after thawing and dilution of simultaneously cryopreserved vaccinal Babesia bovis and Babesia bigemina strains cultured in vitro. Vet Parasitol 61: 345- 348.
34. McElwain TF, Perryman LE, Musoke AJ, McGuire TC (1991) Molecular characterization and immunogenicity of neutralization-sensitive Babesia bigemina merozoite surface proteins. Mol Biochem Parasitol 47: 213-222.
35. Mehla K, Ramana J (2016) Identification of epitope-based peptide vaccine candidates against enterotoxigenic Escherichia coli: a comparative genomics and Immunoinformatics approach. Mol Biosyst 12: 890-901.
36. Menezes-Souza D, Mendes TA, Nagem RA, Santos TT, Silva AL, Santoro MM, de Carvalho SF, Coelho EA, Bartholomeu DC, Fujiwara RT (2014) Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis. PloS One 9: e99216.
37. Mercado-Uriostegui MA, Castro-Sánchez LA, Batiha GE, Valdez-Espinoza UM, Falcón-Neri A, Ramos-Aragon JA, Hernández-Ortiz R, Kawazu SI, Igarashi I, Mosqueda J (2022) The GP-45 Protein, a Highly Variable Antigen from Babesia bigemina, Contains Conserved B-Cell Epitopes in Geographically Distant Isolates. Pathogens 11: 591.
38. Mishra VS, McElwain TF, Dame JB, Stephens EB (1992) Isolation, sequence and differential expression of the p58 gene family of Babesia bigemina. Mol Biochem Parasitol 53: 149-158.
39. Mosqueda J, Olvera-Ramirez A, Aguilar-Tipacamu G, Canto GJ (2012) Current advances in detection and treatment of babesiosis. Curr Med Chem 19: 1504-1518.
40. Moubri K, Kleuskens J, Van de Crommert J, Scholtes N, Van Kasteren T, Delbecq S, Grenflot A, Schetters T (2018) Discovery of a recombinant Babesia canis supernatant antigen that protects dogs against virulent challenge infection. Vet Parasitol 249: 21-29.
41. Mucci J, Carmona SJ, Volcovich R, Altcheh J, Bracamonte E, Marco JD, Nielsen M, Buscaglia CA, Agüero F (2017) Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes. PLoS Negl Trop Dis 11: e0005972.
42. Nabi H, Rashid I, Ahmad N, Durrani A, Akbar H, Islam S, Bajwa AA, Shehzad W, Ashraf K, Imran N (2017) Induction of specific humoral immune response in mice immunized with ROP18 nanospheres from Toxoplasma gondii. Parasitol Res 116: 359-370.
43. Naeem H, Sana M, Islam S, Khan M, Riaz F, Zafar Z, Akbar H, Shehzad W, Rashid MI (2018) Induction of Th1 type-oriented humoral response through intranasal immunization of mice with SAG1- Toxoplasma gondii polymeric nanospheres. Artif Cells Nanomed Biotechnol 46: 1025-1034.
44. Patarroyo MF, Cifuentes G, Bermudez A, Patarroyo MA (2008) Strategies for developing multi‐epitope, subunit‐based, chemically synthesized anti‐malarial vaccines. J Cell Mol Med 12: 1915-1935.
45. Rahman SU, Akbar H, Shabbir MZ, Ullah U, Rashid MI (2021) Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis. Pathogens 10: 1111.
46. Rashid I, Hedhli D, Moiré N, Pierre J, Debierre-Grockiego F, Dimier-Poisson I, Mévélec MN (2011) Immunological responses induced by a DNA vaccine expressing RON4 and by immunogenic recombinant protein RON4 failed to protect mice against chronic toxoplasmosis. Vaccine 29: 8838-8846.
47. Rauf U, Shabir S, Khan M, Rashid MI, Akbar H, Durrani AZ (2020) Identification of 23 kD immunogen from native antigens of Babesia bigemina in splenectomized calf. Int J Agric Biol 24: 1788-1794.
48. Rauf U, Suleman M, Abid A, Jamil H, Menghwar H, Durrani AZ, Rashid MI, Akbar H (2020) Humoral and cell-mediated immune response validation in calves after a live attenuated vaccine of Babesia bigemina. Pathogens 9: 936.
49. Rehman A, Conraths FJ, Sauter‐Louis C, Krücken J, Nijhof AM (2019) Epidemiology of tick‐ borne pathogens in the semi‐arid and the arid agro‐ecological zones of Punjab province, Pakistan. Transbound Emerg Dis 66: 526-536.
50. Rodríguez-Camarillo SD, Quiroz-Castañeda RE, Aguilar-Díaz H, Vara-Pastrana JE, Pescador- Pérez D, Amaro-Estrada I, Martínez-Ocampo F (2020) Immunoinformatic analysis to identify proteins to be used as potential targets to control bovine anaplasmosis. Int J Microbiol 2020: 88.
51. Islam MS, Aryasomayajula A, Selvaganapathy PR (2017) A review on macroscale and microscale cell lysis methods. Micromachines 8: 83
52. Shkap V, Leibovitz B, Krigel Y, Hammerschlag J, Marcovics A, Fish L, Molad T, Savitsky I, Mazuz M (2005) Vaccination of older Bos taurus bulls against bovine babesiosis. Vet Parasitol 129: 235-242.
53. Siddique RM, Sajid MS, Iqbal Z, Saqib M (2020) Association of different risk factors with the prevalence of babesiosis in cattle and buffalos. Pak J Agri Sci 57: 517-524.
54. Silva MG, Knowles DP, Suarez CE (2016) Identification of interchangeable cross-species function of elongation factor-1 alpha promoters in Babesia bigemina and Babesia bovis. Parasit Vectors 9: 576.
55. Singh H, Mishra AK, Rao JR, Tewari AK (2009) Comparison of indirect fluorescent antibody test (IFAT) and slide enzyme linked immunosorbent assay (SELISA) for diagnosis of Babesia bigemina infection in bovines. Trop Anim Health Prod 41: 153- 159.
56. Teimouri A, Modarressi MH, Shojaee S, Mohebali M, Rezaian M, Keshavarz H (2019) Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection of Toxoplasma gondii infections. Infect Drug Resist 12: 2657-2669.
57. Tuvshintulga B, Sivakumar T, Yokoyama N, Igarashi I (2019) Development of unstable resistance to diminazene aceturate in Babesia bovis. Int J Parasitol: Drugs Drug Resist 9: 87-92.
58. Uilenberg G (2006) Babesia—a historical overview. Vet Parasitol 138: 3-10.
59. Wagner GG, Holman P, Waghela S (2002) Babesiosis and heart water: threats without boundaries. Vet Clin North Am Food Anim Pract 18: 417-430.
60. Wieser SN, Schnittger L, Florin-Christensen M, Delbecq S, Schetters T (2019) Vaccination against babesiosis using recombinant GPI-anchored proteins. Int J Parasitol 49: 175-181.
61. Wright IG, Goodger BV, Leatch G, Aylward JH, Rode-Bramanis K, Waltisbuhl DJ (1987) Protection of Babesia bigemina-immune animals against subsequent challenge with virulent Babesia bovis. Infect Immun 55: 364-368.
62. Yadav S, Prakash J, Shukla H, Das KC, Tripathi T, Dubey VK (2020) Design of a multi- epitope subunit vaccine for immune-protection against Leishmania parasite. Pathog Glob Health 114: 471-481.
63. Yoshida M, Reyes SG, Tsuda S, Horinouchi T, Furusawa C, Cronin L (2017) Time- programmable drug dosing allows the manipulation, suppression and reversal of antibiotic drug resistance in vitro. Nat Commun 8: 15589.