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Abstract

This paper presents an identification method of pollution inflow directions based on the circular graphs of percentiles of pollution concentrations and the possibilities of applying these graphs to estimation of pollutant emission. Based on these graphs of particulate matter concentration recorded in winter and summer seasons, the inflows of dust from the direction of fly ash landfill have been compared for summer and winter periods. This analysis enables to assess the relation of concentrations of pollution from secondary particulate matter suspension to concentrations of pollution generated by other sources. This paper also argues that the 24-hours concentrations, though commonly used, may prove to be an indicator of minor importance to assess air pollution status. On the one hand, excessive 24-hours concentrations impede the identification of the polluter, on the other hand, concentrations generally within permissible limits may occasionally peak dangerously at some sources. As an example, an existing case and a numerical experiment have been presented.
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Authors and Affiliations

Czesław Kliś
Marek Matejczyk
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Abstract

This study represents the first culture-independent profiling of microbial diversity in post-processing wastewater from underground coal gasification (UCG) processes. Three types of post-processing wastewater, named W1, W2 and W3, were obtained from three UCG processes involving two types of coal and two gasification agents, namely oxygen-enriched air and oxygen. Very high concentrations of BTEX (benzene, toluene, ethylbenzene, xylene), polyaromatic hydrocarbons (PAHs), and phenol were detected in the wastewater, classifying it into the fifth toxicity class, indicating very high acute toxicity. The values for the Shannon (H), Ace and Chao1 indices in W2 were the lowest compared to their values in W1 and W3. The dominate phyla were Proteobacteria, contributing 84.64% and 77.92% in W1 and W3, respectively, while Firmicutes dominated in W2 with a contribution of 66.85%. At the class level, Gammaproteobacteria and Alphaproteobacteria were predominant in W1 and W3, while Bacilli and Actinobacteria were predominant in W2. Among Bacilli, the Paenibacillus and Bacillus genera were the most numerous. Our results suggest that the main differentiating factor of the bacterial structure and diversity in the wastewater could be the gasification agent. These findings provide new insights into the shifting patterns of dominant bacteria in post-processing wastewater and illustrate the spread of bacteria in industrial contaminated wastewater.
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Bibliography

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  22. Persoone, G., Marsalek, B., Blinova, I., Torokne, A., Zarina, D., Manusadzianas, L. (2003). A practical and user-friendly toxicity classification system with microbiotests for natural waters and wastewaters, Environmental Toxicology, 18, pp. 395–402. DOI:10.1002/tox.10141.
  23. Rappaport, H.B. & Oliverio, A.M. (2023). Extreme environments offer an unprecedent opportunity to understand microbial eukaryotic ecology, evolution, and genome biology, Nature Communication, 14, 4959. DOI:10.1038/s41467-023-40657-4
  24. Sharma, S. & Bhattacharya, A. (2017) Drinking water contamination and treatment techniques. Appied Water Science 7, pp. 1043-1067. DOI:10.1007/s13201-016-0455-7
  25. Smoliński, A.. Stańczyk, K.. Kapusta, K. & Howaniec, N. (2013). Analysis of the organic contaminants in the condensate produced in the in situ underground coal gasification process, Water Science and Technology, 67, pp. 644-650. DOI:10.2166/wst.2012.558
  26. Thukral, A.K. (2017). A review on measurement of alpha diversity in biology, Agricultural Research Journal, 54, 1. DOI:10.5958/2395-146X.2017.00001.1
  27. Timkina, E., Drabova, L., Palyova, A,, Rezanka, T., Matatkova, O. & Kolouchova, I. (2020). Kocuria strains from unique radon spring water from Jachymov Spa, Fermentation, 8, 35. DOI:10.3390/fermentation8010035
  28. Wiatowski, M., Kapusta, K., Strugała-Wilczek, A., Stańczyk, K., Castro-Muñiz, A., Suárez-García F. & Paredes, J.I. (2023). Large-scale experimental simulations of in situ coal gasification in terms of process efficiency and physicochemical properties of process by-products, Energies, 16, 4455. DOI:10.3390/en16114455
  29. Xu, B., Chen, L., Xing, B., Li, Z., Zhang, L., Yi, G., Huang, G. & Mohanty, M.K. (2017). Physicochemical properties of Hebi semi-coke from underground coal gasification and its adsorption for phenol, Process Safety Environmental Protection, 107, pp. 147–152. DOI:10.1016/j.psep.2017.02.007
  30. Yang, Y., Wang, L., Xiang, F., Zhao, L. & Qiao, Z. (2020). Activated sludge microbial community and treatment performance of wastewater treatment plants in industrial and municipal zones, International Journal of Environmental Research and Public Health, 17, 436. DOI:10.3390/ijerph17020436
  31. Zwain, H., Al-Marzook, F., Nile, B., Ali Jeddoa, Z., Atallah, A., Dahlan, I. & Hassan, W. (2021). Morphology analysis and microbial diversity in novel anaerobic baffled reactor treating recycled paper mill wastewater, Archives of Environmental Protection, 47, 4, pp. 9–17. DOI:10.24425/aep.2021.139498
  32. Bassin, J.; Rachid, C.; Vilela, C. Cao, S.; Peixoto, R. & Dezotti, M. (2017). Revealing the bacterial profile of an anoxic-aerobic moving-bed biofilm reactor system treating a chemical industry wastewater, International Biodeterioration & Biodegradation, 120, pp. 152–160. DOI:10.1016/j.ibiod.2017.01.036
  33. Bedogni, G.L.; Massello, F. L.; Giaveno, A.; Donati, E.R. & Urbieta, M.S. (2020). A deeper look into the biodiversity of the extremely acidic copahue volcano - Río Agrio system in Neuquén, Argentina, Microorganisms, 8, 58. DOI:10.3390/microorganisms8010058
  34. Chen, T.; Wu, Y.; Wang, J. & Philippe, C. F. X. (2022). Assessing the biodegradation of btex and stress response in a bio-permeable reactive barrier using compound-specific isotope analysis, International Journal of Environmental Research and Public Health, 19, 8800. DOI:10.3390/ijerph19148800
  35. Fimlaid, K. A. & Shen, A. (2015). Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes, Current Opinion in Microbiology, 24, pp. 88-95. DOI:10.1016%2Fj.mib.2015.01.006
  36. Gawroński, S., Łutczyk, G.; Szulc, W. & Rutkowska, B. (2022). Urban mining: Phytoextraction of noble and rare earth elements from urban soils, Archives of Environmental Protection, 48, 2, pp. 24-33. DOI:10.24425/aep.2022.140763
  37. Grabowski, J., Korczak, K. & Tokarz, A. (2021). Aquatic risk assessment based on the results of research on mine waters as a part of a pilot underground coal gasification process, Process Safety and Environmental Protection, 148, pp. 548-558. DOI:10.1016/j.psep.2020.10.003
  38. Grady, E.N., MacDonald, J., Richman, A. & Yuan, Z.C. (2016). Current knowledge and perspectives of Paenibacillus: a review. Microbial Cell Factories, 15, 203. DOI:10.1186/s12934-016-0603-7
  39. Guisado, I.M., Purswani, J., Gonzales-Lopez, J. & Pozo, C. (2015). Physiological and genetic screening methods for isolation of methyl-tert-butyl-ether-degrading bacteria for bioremediation purposes, International Biodeterioration and Biodegradation, 97, pp. 67-74. DOI:10.1016/j.ibiod.2014.11.008
  40. Jałowiecki, Ł., Borgulat, J.; Strugała-Wilczek, A., Glaser, M. & Płaza, G. (2024). Searching of phenol-degrading bacteria in raw wastewater from underground coal gasification process as suitable candidates in bioaugmentation approach, Journal of Ecological Engineering, 25, pp. 62–71. DOI:10.12911/22998993/176143
  41. Jayapal, A., Chaterjee, T. & Sahariah, B.P. (2023). Bioremediation techniques for the treatment of mine tailings: A review, Soil Ecology Letters, 5, 220149. DOI:10.1007/s42832-022-0149-z
  42. Kamika, I., Azizi, S. & Tekere, M. (2016). Microbial profiling of South African acid mine water samples using next generation sequencing platform, Applied. Microbiology and Biotechnology, 100, pp.6069–6079. DOI:10.1007/s00253-016-7428-5
  43. Kapusta, K. & Stańczyk, K. (2015). Chemical and toxicological evaluation of underground coal gasification (UCG) effluents. The coal rank effect, Ecotoxicology and Environmental Safety, 112, pp. 105– 113. DOI:10.1016/j.ecoenv.2014.10.038
  44. Karn, S.K., Chakrabarti, S.K. & Reddy, M.S. (2011). Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill, Biodegradation, 22, pp. 63-69. DOI:10.1007/s10532-010-9376-6
  45. Kochhar, N., Kavya, I.K., Shrivvastava, S., Ghosh, A., Rawat, V.S., Sodhi, K.K. & Kumar, M. (2022) Perspectives on the microorganisms of extreme environments and their applications, Current Research Microbial Sciences. 3, 100134. DOI:10.1016/j.crmicr.2022.100134
  46. Liu, F., Hu, X., Zhao, X., Guo, H. & Zhao, Y. (2019). Microbial community structures’ response to seasonal variation in a full-scale municipal wastewater treatment plant, Environmental Engineering Science, 36, pp. 172-178. DOI:10.1089/ees.2018.0280
  47. Luo, Z., Ma, J., Chen, F., Li, X., Zhang, Q. & Yang, Y. (2020). Adaptive development of soil bacterial communities to ecological processes caused by mining activities in the Loess Plateau, China, Microorganisms, 8, 477. DOI:10.3390/microorganisms8040477
  48. Mauricio-Gutiérrez, A., Machorro-Velázquez R., Jiménez-Salgado, T.;Vázquez-Crúz C., Sánchez-Alonso, M.P. & Tapia-Hernández, A. (2020). Bacillus pumilus and Paenibacillus lautus effectivity in the process of biodegradation of diesel isolated from hydrocarbons contaminated agricultural soils, Archives of Environmental Protection, 46, 4, pp. 59–69. DOI:0.24425/aep.2020.135765
  49. Muter, O. (2023). Current trends in bioaugmentation tools for bioremediation: A critical review of advances and knowledge gaps, Microorganisms, 11, 710. DOI:10.3390/microorganisms11030710
  50. Nwankwegu, A.S., Zhang, L., Xie, D., Onwosi, C.O., Muhammad, W.I., Odoh, C.K., Sam, K. & Idenyi, J.N. (2022). Bioaugmentation as a green technology for hydrocarbon pollution remediation. Problems and prospects. Journal of Environmental Management, 304, 114313. DOI:10.1016/j.jenvman.2021.114313
  51. Pankiewicz-Sperka, M., Kapusta, K., Basa, W. & Stolecka, K. (2021). Characteristics of water contaminants from underground coal gasification (UCG) process - effect of coal properties and gasification pressure, Energies, 14, 6533. DOI:10.3390/en14206533
  52. Pankiewicz-Sperka, M., Stańczyk, K., Płaza, G., Kwaśniewska, J. & Nałęcz-Jawecki, G. (2014). Assessment of the chemical, microbiological and toxicological aspects pf post-processing water from underground coal gasification, Ecotoxicology and Environmental Safety, 108, pp. 294-301. DOI:10.1016/j.ecoenv.2014.06.036
  53. Persoone, G., Marsalek, B., Blinova, I., Torokne, A., Zarina, D., Manusadzianas, L. (2003). A practical and user-friendly toxicity classification system with microbiotests for natural waters and wastewaters, Environmental Toxicology, 18, pp. 395–402. DOI:10.1002/tox.10141.
  54. Rappaport, H.B. & Oliverio, A.M. (2023). Extreme environments offer an unprecedent opportunity to understand microbial eukaryotic ecology, evolution, and genome biology, Nature Communication, 14, 4959. DOI:10.1038/s41467-023-40657-4
  55. Sharma, S. & Bhattacharya, A. (2017) Drinking water contamination and treatment techniques. Appied Water Science 7, pp. 1043-1067. DOI:10.1007/s13201-016-0455-7
  56. Smoliński, A.. Stańczyk, K.. Kapusta, K. & Howaniec, N. (2013). Analysis of the organic contaminants in the condensate produced in the in situ underground coal gasification process, Water Science and Technology, 67, pp. 644-650. DOI:10.2166/wst.2012.558
  57. Thukral, A.K. (2017). A review on measurement of alpha diversity in biology, Agricultural Research Journal, 54, 1. DOI:10.5958/2395-146X.2017.00001.1
  58. Timkina, E., Drabova, L., Palyova, A,, Rezanka, T., Matatkova, O. & Kolouchova, I. (2020). Kocuria strains from unique radon spring water from Jachymov Spa, Fermentation, 8, 35. DOI:10.3390/fermentation8010035
  59. Wiatowski, M., Kapusta, K., Strugała-Wilczek, A., Stańczyk, K., Castro-Muñiz, A., Suárez-García F. & Paredes, J.I. (2023). Large-scale experimental simulations of in situ coal gasification in terms of process efficiency and physicochemical properties of process by-products, Energies, 16, 4455. DOI:10.3390/en16114455
  60. Xu, B., Chen, L., Xing, B., Li, Z., Zhang, L., Yi, G., Huang, G. & Mohanty, M.K. (2017). Physicochemical properties of Hebi semi-coke from underground coal gasification and its adsorption for phenol, Process Safety Environmental Protection, 107, pp. 147–152. DOI:10.1016/j.psep.2017.02.007
  61. Yang, Y., Wang, L., Xiang, F., Zhao, L. & Qiao, Z. (2020). Activated sludge microbial community and treatment performance of wastewater treatment plants in industrial and municipal zones, International Journal of Environmental Research and Public Health, 17, 436. DOI:10.3390/ijerph17020436
  62. Zwain, H., Al-Marzook, F., Nile, B., Ali Jeddoa, Z., Atallah, A., Dahlan, I. & Hassan, W. (2021). Morphology analysis and microbial diversity in novel anaerobic baffled reactor treating recycled paper mill wastewater, Archives of Environmental Protection, 47, 4, pp. 9–17. DOI:10.24425/aep.2021.139498
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Authors and Affiliations

Łukasz Jałowiecki
1
Jacek Borgulat
1
Aleksandra Strugała-Wilczek
2
Jan Jastrzębski
3
Marek Matejczyk
1
Grażyna Płaza
4

  1. Institute for Ecology of Industrial Areas,Katowice, Poland
  2. Department of Energy Saving and Air Protection, Central Mining Institute, Katowice, Poland
  3. Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
  4. Silesian University of Technology, Poland

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