Chromosome numbers of 46 Hieracium L. and Pilosella Vaill. taxa from Austria, Bulgaria, Czech Republic, Macedonia, Montenegro, Poland, Romania, Serbia and Slovakia are presented. Chromosomes numbers are given for the first time for Hieracium amphigenum Briq. 2n = 3× = 27, H. bohatschianum Zahn 2n = 4× = 36, H. borbasii R. Uechtr. 2n = 4× = 36, H. cernuum Friv. 2n = 2× = 18, H. hazslinszkyi Pax 2n = 3× = 27, H. mirekii Szeląg 2n = 4× = 36, H. polyphyllobasis (Nyár. & Zahn) Szeląg 2n = 3× = 27, H. porphyriticum A. Kern. 2n = 4× = 36, H. racemosum Waldst. & Kit. ex Willd. subsp. racemosum 2n = 3× = 27, H. scardicum Borm. & Zahn 2n = 4× = 36, H. sparsum subsp. ipekanum Rech. fil. & Zahn 2n = 4× = 36, H. sparsum subsp. peristeriense Behr & Zahn, H. sparsum subsp. squarrosobracchiatum Behr & al. 2n = 3× = 27, H. tomosense Simk. 2n = 4× = 36, H. tubulare Nyár. 2n = 4× = 36, H. werneri Szeląg 2n = 3× = 27 and Pilosella fusca subsp. subpedunculata (Zahn) Szeląg, as well as five species of Hieracium sect. Cernua R. Uechtr. not described to date and a hybrid between H. bifidum s. lat. and H. pojoritense Woł