In performed experiments, insoluble polyvinylpolypyrrolidone, PVPP as an additive to the extraction buffer was used for isolation of total nucleic acids from hop plants and grapevine in order to obtain templates useful for detection ofHLVd and HSVd by means ofRT-PCR. Addition of2% of PVPP to the original GTC buffer (Chomczynski and Sacchi, 1987) appeared to be the most favorable. Due to PVPP addition, the protocol of extraction of nucleic acids was simplified by shortening of isolation time and reduction of expenses. However, application of the simplified method for obtaining of templates that guaranteed full repeatability of test results was limited to the spring and early summer season.