Investigations on the occurrence of Barley stripe mosaic virus (BSMV, Hordeivirus) in Poland were performed by testing seeds of 22 barley cultivars. BSMV was detected in seeds of winter barley cv. Tiffany and of spring barley cvs. Scarlett and Stratu s. The virus presence was revealed by ELISA test and then confirm ed by electron microscopy. Preliminary data on the rate of seed transmission of BSMV in cvs. Scarlett and Stratus are presented.