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Abstract

A measurement system for 256-channel in vitro recordings of brain tissue electrophysiological activity is presented in the paper. The system consists of the brain tissue life support system, Microelectrode Array (MEA), conditioning Application Specific Integrated Circuits (ASIC’s) for signals conditioning, Digitizer and PC application for measurement data presentation and storage. The life support system keeps brain tissue samples in appropriately saturated artificial cerebrospinal fluid at a very stable temperature. The MEA consists of two hundred and fifty-six 40 μm diameter tip-shaped electrodes. The ASIC’s performs amplification and filtering of the 256-field and action potential signals. The Digitizer performs simultaneous data acquisition from 256 channels 14 kS/s sample rate and 12-bit resolution. The resulting byte stream is transmitted to the PC via USB (Universal Serial Bus). Preliminary tests confirm that the system is capable of keeping the extracted brain tissue active (hippocampal formation slices) and simultaneously to record action potentials, as well as local theta field potentials with very small amplitudes from multiple neurons

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Authors and Affiliations

Miroslaw Zoladz
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Abstract

In the paper, the results of investigations on the properties of acoustic emission signals generated in a tested pressure vessel are presented. The investigations were performed by repeating several times the following procedure: an increase in pressure, maintaining a given pressure level, a further increase in pressure, and then maintaining the pressure at new determined level. During the tests the acoustic emission signals were recorded by the measuring system 8AE-PD with piezoelectric sensors D9241A. The used eight-channel measuring system 8AE-PD enables the monitoring, recording and then basic and advanced analysis of signals.

The results of basic analysis carried out in domain of time and the results of advanced analysis carried out in the discrimination threshold domain of the recorded acoustic emission signals are presented in the paper.

In the framework of the advanced analysis, results are described by the defined by the author descriptors with acronyms ADC, ADP and ADNC. Such description is based on identifying the properties of amplitude distributions of acoustic emission signals by assigning them the level of advancement. It is shown that for signals including continoues AE or single burst AE signals descriptions of such registered signals by means of ADC, ADP and ADNC descriptors and by Upp and Urms descriptors provide identical ordering of registered acoustic emission signals. For complex signals, the description using ADC, ADP and ADNC descriptors based on the analysis of amplitude distributions of recorded signals gives the order of signals with more accurate connection with deformational processes being sources of acoustic emission signals.

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Authors and Affiliations

Franciszek Witos

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