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Abstract

The purpose of this research was to determine: 1) phosphate bacteria (PB) mechanisms responsible for enhancing the soil’s soluble phosphorus (P), using the processes of inorganic P solubilisation, organic P mineralization, and blocking of soil colloidal adsorption site, and 2) to investigate the factors contributing to this increase. Phosphate bacteria (PB) was inoculated into sterile Andisols in three separate compositions, termed 1 kg P∙kg–1 (2.82 g phosphate rock, 0.5 g Ca3(PO4)2, 0.4 g Al3(PO4)2, or 0.4 g Fe3(PO4)2), organic P (0.5 cm3 para-Nitrophenylphosphate (pNPP) or 0.5 g Na-phytate), and 1 kg P (KH2PO4)∙kg–1, in order to analyse inorganic P solubilisation, organic P mineraliza-tion, and evaluate blocking soil colloidal site for adsorption P, respectively. Furthermore, spectrophotometry technique was applied to determine the amount of dissolved P. The PB showed an improvement in inorganic P solubilisation from 147.66 to 194.61 mg P∙kg–1, and also in organic P from 63.6 to 91.7 mg P∙kg–1, compared to control, (31.06 mg P∙kg–1) and (23.7 mg P∙kg–1), respectively. Meanwhile, the micro-organisms were known to decrease P adsorption by 13.43%, beyond the restraint set at 85.34%. Therefore, increased soluble P in Andisols is possibly expressed, using the equation as follows: soluble P (mg P∙kg–1) = 1201.96 + 1.18 inorganic P solubilisation (mg P∙kg–1) + 1.09 organic P mineralization (mg P∙kg–1) – 0.92 adsorption P (mg P∙kg–1) (R2 = 0.99).
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Authors and Affiliations

Tamad
1
Azwar Maas
2
Eko Hanudin
2
Jaka Widada
2

  1. Jenderal Soedirman University, Faculty of Agriculture, Agrotechnology Department, dr. Soeparno Street Purwokerto 53123, Central of Java, Indonesia
  2. Gadjah Mada University, Faculty of Agriculture, Soil Science Department, Flora Street Bulaksumur 55281, Yogyakarta, Indonesia

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