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Abstract

Porcine circovirus type 2 (PCV2) is an economically important swine pathogen and, although small, it has the highest evolution rate among DNA viruses. Commercial PCV2 commercial vaccines are inactivated PCV2 isolates or a subunit vaccine based on the Cap protein of PCV2. Currently, PCV2 VLPs of individual subtype vaccines are available. Although the main prevalent genotype worldwide is PCV2b, the emerging subtype PCV2d subtype is also increasingly associated with PCV disease. The aim of the study was to evaluate the protective efficacy of VLP based on the PCV2b and 2d subtypes against the mixed challenge of two hypotype PCV2 in mice. Thirty-six female SPV Kunming mice were immunized twice with PCV2b and 2d VLPs, then challenged with PCV2b and PCV2d, to assess the immunogenicity and effectiveness of the VLPs. Vaccination of the mice with PCV2b and 2d VLPs elicited a robust antibody response specific for the PCV2. The virus load detected in the 2b and 2d spleen vaccine group was the lowest compared to other groups. Furthermore, there was no pathological damage in the HE stained sections of the 2b and 2d spleen vaccine, and no virus was detected in the immunohistochemical sections. Our data suggest that the mixed PCV2b and 2d VLP vaccine could protect mice from challenge with the mixed infection of PCV2b and PCV2d.
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Bibliography


Akahata W, Yang ZY, Andersen H, Sun S, Holdaway HA, Kong WP, Lewis MG, Higgs S, Rossmann MG, Rao S, Nabel GJ (2010) A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection. Nat Med 16: 334-338.
Firth C, Charleston MA, Duffy S, Shapiro B, Holmes EC (2009) Insights into the evolutionary history of an emerging livestock pathogen: porcine circovirus 2. J Virol 83: 12813-12821.
Forstova J, Krauzewicz N, Wallace S, Street A J, Dilworth S M, Beard S, Griffin B E (1993) Cooperation of structural proteins during late events in the life cycle of polyomavirus. J Virol 67: 1405-1413.
Fraile L, Sibila M, Nofrarias M, Lopez-Jimenez R, Huerta E, Llorens A, Lopez-Soria S, Perez D, Segales J (2012) Effect of sow and piglet porcine circovirus type 2 (PCV2) vaccination on piglet mortality, viraemia, antibody titre and production parameters. Vet Microbiol 161: 229-234.
Ge X, Wang F, Guo X, Yang H (2012) Porcine circovirus type 2 and its associated diseases in China. Virus Res 164: 100-106.
Guo L, Fu Y, Wang Y, Lu Y, Wei Y, Tang Q, Fan P, Liu J, Zhang L, Zhang F, Huang L, Liu D, Li S, Wu H, Liu C (2012) A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain. PLoS One 7: e41463.
Guo LJ, Lu YH, Wei YW, Huang LP, Liu CM (2010) Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China. Virol J 7: 273.
Hemann M, Beach NM, Meng XJ, Halbur PG, Opriessnig T (2012) Vaccination with inactivated or live-attenuated chimeric PCV1-2 results in decreased viremia in challenge-exposed pigs and may reduce transmission of PCV2. Vet Microbiol 158: 180-186.
Jiang W, Li M, He F, Zhou S, Zhu L (2017) Targeting the NLRP3 inflammasome to attenuate spinal cord injury in mice. J Neuroinflammation 14: 207.
Jourdan N, Godeke G J, Penaud M, Mottola G, Sorrentino A, Rottier P J, Bonatti S (2006) Assembly of HCV E1 and E2 glycoproteins into coronavirus VLPs. Arch Virol 151: 2085-2094.
Kixmoller M, Ritzmann M, Eddicks M, Saalmuller A, Elbers K, Fachinger V (2008) Reduction of PMWS-associated clinical signs and co-infections by vaccination against PCV2. Vaccine 26: 3443-3451.
Kwon T, Lee DU, Yoo SJ, Je SH, Shin JY, Lyoo YS (2017) Genotypic diversity of porcine circovirus type 2 (PCV2) and genotype shift to PCV2d in Korean pig population. Virus Res 228: 24-29.
Mohsen MO, Zha L, Cabral-Miranda G, Bachmann MF (2017) Major findings and recent advances in virus- -like particle (VLP)-based vac-cines. Semin Immunol 34: 123-132.
Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS (2000) Open reading frame 2 of porcine circo- virus type 2 encodes a major capsid protein. J Gen Virol 81: 2281-2287.
Opriessnig T, Shen HG, Pal N, Ramamoorthy S, Huang YW, Lager KM, Beach NM, Halbur PG, Meng XJ (2011) A live-attenuated chimeric porcine circovirus type 2 (PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (PPV) and porcine reproductive and respira- tory syndrome virus (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model. Clin Vaccine Immunol 18: 1261-1268.
Palkova Z, Adamec T, Liebl D, Stokrova J, Forstova J (2000) Production of polyomavirus structural protein VP1 in yeast cells and its interac-tion with cell structures. FEBS Lett 478: 281-289.
Ramqvist T, Andreasson K, Dalianis T (2007) Vaccination, immune and gene therapy based on virus-like particles against viral infections and cancer. Expert Opin Biol Ther 7: 997-1007.
Salunke DM, Caspar DL, Garcea RL (1986) Self-assembly of purified polyomavirus capsid protein VP1. Cell 46: 895-904.
Segales J (2015) Best practice and future challenges for vaccination against porcine circovirus type 2. Expert Rev Vaccines 14: 473-487.
Segales J, Calsamiglia M, Olvera A, Sibila M, Badiella L, Domingo M (2005) Quantification of porcine circovirus type 2 (PCV2) DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without postweaning multisystemic wasting syndrome (PMWS). Vet Microbiol 111: 223-229.
Segales J, Martinez-Guino L, Cortey M, Navarro N, Huerta E, Sibila M, Pujols J, Kekarainen T (2009) Retrospective study on swine Torque teno virus genogroups 1 and 2 infection from 1985 to 2005 in Spain. Vet Microbiol 134(3-4): 199-207.
Stewart M, Bhatia Y, Athmaran TN, Noad R, Gastaldi C, Dubois E, Russo P, Thiery R, Sailleau C, Breard E, Zientara S, Roy P (2010) Vali-dation of a novel approach for the rapid production of immunogenic virus-like particles for bluetongue virus. Vaccine 28: 3047-3054.
Szecsi J, Boson B, Johnsson P, Dupeyrot-Lacas P, Matrosovich M, Klenk HD, Klatzmann D, Volchkov V, Cosset FL (2006) Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. Virol J 3: 70.
Wang F, Guo X, Ge X, Wang Z, Chen Y, Cha Z, Yang H (2009) Genetic variation analysis of Chinese strains of porcine circovirus type 2. Virus Res 145: 151-156.
Xiao CT, Halbur PG, Opriessnig T (2012) Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vac-cine failures in the United States. J Virol 86: 12469.
Xiao CT, Halbur PG, Opriessnig T (2015) Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d. J Gen Virol 96: 1830-1841.
Xiao CT, Harmon KM, Halbur PG, Opriessnig T (2016) PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014-2016. Vet Microbiol 197: 72-77.
Yang S, Yin S, Shang Y, Liu B, Yuan L, Zafar Khan MU, Liu X, Cai J (2018) Phylogenetic and genetic variation analyses of porcine circovirus type 2 isolated from China. Transbound Emerg Dis 65: e383-e392.
Yuan X, Lin H, Li B, He K, Fan H (2017) Efficacy and immunogenicity of recombinant swinepox virus expressing the truncated S protein of a novel isolate of porcine epidemic diarrhea virus. Arch Virol 162: 3779-3789.
Zhang Y, Wang Z, Zhan Y, Gong Q, Yu W, Deng Z, Wang A, Yang Y, Wang N (2016) Generation of E. coli-derived virus-like particles of porcine circovirus type 2 and their use in an indirect IgG enzyme-linked immunosorbent assay. Arch Virol 161: 1485-1491.
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Authors and Affiliations

X.M. Yuan
1
Q.C. Yuan
1
S.M. Feng
1
Z.B. Deng
1

  1. Laboratory of Animal Disease Prevention and Control and Animal model, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, No. 1 Nongda road, Furong District, Changsha, 410128, People’s Republic of China
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Abstract

Porcine parvovirus (PPV) is a major causative agent in reproductive pig disease. The swine industry faces a significant economic and epizootic threat; thus, finding a reliable, quick, and practical way to detect it is essential. In this investigation, recombinant PPV VP2 protein was expressed in the Escherichia coli ( E. coli) expression systems. As shown by electron microscopy (TEM), Western blot, and hemagglutination (HA) assays, the recombinant VP2 protein was successfully assembled into virus-like particles (VLPs) after being expressed and purified. These VLPs had a structure that was similar to that of real PPV viruses and also exhibited HA activity. These VLPs induced high levels of PPV-specific antibody titers in mice after immunization, indicating that the VLPs may be beneficial as potential candidate antigens. VLPs were used as the coating antigens for the VLP ELISA, and the PPV VLPs-based ELISA displayed a high sensitivity (99%), specificity (93.0%) and agreement rate (98.3%) compared to HI assay, and the agreement rate of this ELISA was 97.5% compared to a commercial ELISA kit. Within a plate, the coefficient of variation (CV) was 10%, and between ELISA plates, the CV was 15%. According to a cross-reactivity assay, the technique was PPV-specific in contrast to other viral illness sera. The PPV VLP indirect-ELISA test for PPV detection in pigs with an inactivated vaccine showed that the PPV-positive rate varied among different sample sources from 88.2 to 89.6%. Our results indicate that this ELISA technique was quick, accurate, and repeatable and may be used for extensive serological research on PPV antibodies in pigs.
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Bibliography

1. Allan GM, Kennedy S, McNeilly F, Foster JC, Ellis JA, Krakowka SJ, Meehan BM, Adair BM (1999) Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121(1): 1-11.
2. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B (2005) Cloning of a human parvovirus by molecu-lar screening of respiratory tract samples. Proc Natl Acad Sci U S A 102(36): 12891-12896.
3. Cartwright SF, Lucas M, Huck RA (1971) A small haemaggultinating porcine DNA virus. II. Biological and serological studies. J Comp Pathol 81(1): 145-155.
4. Choi C, Chae C (2000) Distribution of porcine parvovirus in porcine circovirus 2-infected pigs with postweaning multisystemic wasting syndrome as shown by in-situ hybridization. J Comp Pathol 123(4): 302-305.
5. Crowther JR (2000) The ELISA guidebook. Methods Mol Biol 149: III-IV, 1-413.
6. Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, Martin K, McNeilly F (2000) Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystem-ic wasting syndrome. J Vet Diagn Invest 12(1): 21-27.
7. Feng H, Hu GQ, Wang HL, Liang M, Liang H, Guo H, Zhao P, Yang YJ, Zheng XX, Zhang ZF, Zhao YK, Gao YW, Yang ST, Xia XZ (2014) Canine parvovirus VP2 protein expressed in silkworm pupae self-assembles into virus-like particles with high immunogenic-ity. PLoS One 9(1): e79575.
8. Hohdatsu T, Baba K, Ide S, Tsuchimoto M, Nagano H, Yamagami T, Yamagishi H, Fujisaki Y, Matumoto M (1988) Detection of anti-bodies against porcine parvovirus in swine sera by enzyme-linked immunosorbent assay. Vet Microbiol 17(1): 11-19.
9. Hua T, Zhang D, Tang B, Chang C, Liu G, Zhang X (2020) The immunogenicity of the virus-like particles derived from the VP2 protein of porcine parvovirus. Vet Microbiol 248: 108795.
10. Jenkins CE (1992) An enzyme-linked immunosorbent assay for detection of porcine parvovirus in fetal tissues. J Virol Methods 39(1-2): 179-184.
11. Ji P, Liu Y, Chen Y, Wang A, Jiang D, Zhao B, Wang J, Chai S, Zhou E, Zhang G (2017) Porcine parvovirus capsid protein expressed in Escherichia coli self-assembles into virus-like particles with high immunogenicity in mice and guinea pigs. Antiviral Res 139: 146-152.
12. Joo HS, Donaldson-Wood CR, Johnson RH (1976) A standardised haemagglutination inhibition test for porcine parvovirus antibody. Aust Vet J 52(9): 422-424.
13. Jozwik A, Manteufel J, Selbitz HJ, Truyen U (2009) Vaccination against porcine parvovirus protects against disease, but does not pre-vent infection and virus shedding after challenge infection with a heterologous virus strain. J Gen Virol 90(Pt 10): 2437-2441.
14. Kennedy S, Moffett D, McNeilly F, Meehan B, Ellis J, Krakowka S, Allan GM (2000) Reproduction of lesions of postweaning multi-systemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvo-virus. J Comp Pathol 122(1): 9-24.
15. Kong M, Peng Y, Cui Y, Chang T, Wang X, Liu Z, Liu Y, Zhu Y, Luo Y, Tang Q, Feng L, Cui S (2014) Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus. J Virol Methods 206: 115-118.
16. Marcekova Z, Psikal I, Kosinova E, Benada O, Sebo P, Bumba L (2009) Heterologous expression of full-length capsid protein of por-cine circovirus 2 in Escherichia coli and its potential use for detection of antibodies. J Virol Methods 162(1-2): 133-141.
17. Mengeling WL, Cutlip RC (1976) Reproductive disease experimentally induced by exposing pregnant gilts to porcine parvovirus. Am J Vet Res 37(12): 1393-1400.
18. Mengeling WL, Lager KM, Vorwald AC (2000) The effect of porcine parvovirus and porcine reproductive and respiratory syndrome vi-rus on porcine reproductive performance. Anim Reprod Sci 60-61: 199-210.
19. Meszaros I, Olasz F, Csagola A, Tijssen P, Zadori Z (2017) Biology of porcine parvovirus (Ungulate parvovirus 1). Viruses 9(12): 393.
20. Oravainen J, Hakala M, Rautiainen E, Veijalainen P, Heinonen M, Tast A, Virolainen JV, Peltoniemi OA (2006) Parvovirus antibodies in vaccinated gilts in field conditions-results with HI and ELISA tests. Reprod Domest Anim 41(1): 91-93.
21. Oravainen J, Heinonen M, Tast A, Virolainen J, Peltoniemi O (2005) High porcine parvovirus antibodies in sow herds: prevalence and associated factors. Reprod Domest Anim 40(1): 57-61.
22. Qing L, Lv J, Li H, Tan Y, Hao H, Chen Z, Zhao J, Chen H (2006) The recombinant nonstructural polyprotein NS1 of porcine parvovi-rus (PPV) as diagnostic antigen in ELISA to differentiate infected from vaccinated pigs. Vet Res Commun 30(2): 175-190.
23. Roic B, Cajavec S, Toncic J, Madic J, Lipej Z, Jemersic L, Lojkic M, Mihaljevic Z, Cac Z, Sostaric B (2005) Prevalence of antibodies to porcine parvovirus in wild boars (Sus scrofa) in Croatia. J Wildl Dis 41(4): 796-799.
24. Shang SB, Li YF, Guo JQ, Wang ZT, Chen QX, Shen HG, Zhou JY (2008) Development and validation of a recombinant capsid pro-tein-based ELISA for detection of antibody to porcine circovirus type 2. Res Vet Sci 84(1): 150-157.
25. Streck AF, Canal CW, Truyen U (2015) Molecular epidemiology and evolution of porcine parvoviruses. Infect Genet Evol 36: 300-306.
26. Westenbrink F, Veldhuis MA, Brinkhof JM (1989) An enzyme-linked immunosorbent assay for detection of antibodies to porcine par-vovirus. J Virol Methods 23(2): 169-178.
27. Xu Y, Li Y (2007) Induction of immune responses in mice after intragastric administration of Lactobacillus casei producing porcine par-vovirus VP2 protein. Appl Environ Microbiol 73(21): 7041-7047.
28. Zeeuw EJL, Leinecker N, Herwig V, Selbitz HJ, Truyen U (2007) Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts. J Gen Virol 88(Pt 2): 420-427.
29. Zheng HH, Wang LQ, Fu PF, Zheng LL, Chen HY, Liu F (2020) Characterization of a recombinant pseudorabies virus expressing por-cine parvovirus VP2 protein and porcine IL-6. Virol J 17(1): 19.
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Authors and Affiliations

Y. Li
1
Q. Wang
2
W. Yue
1
X. Li
1
Y. Chen
1
Y. Gao
1

  1. Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China
  2. State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

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