@ARTICLE{Wierzchowski_Kamil_Propagation_2019, author={Wierzchowski, Kamil and Grabowska, Iwona and Pilarek, Maciej}, volume={vol. 40}, number={No 2}, journal={Chemical and Process Engineering}, pages={167-177}, howpublished={online}, year={2019}, publisher={Polish Academy of Sciences Committee of Chemical and Process Engineering}, abstract={Typically applied static (i.e. non-agitated) cultures do not provide sufficient conditions for efficient propagation of suspended non-adherent cells, in general. Feasibility of small-scale wave-type agitated single-use bioreactors for gentle agitation underlies applicability of such systems for scaling-up of fragile biomass of animal cells. The basic aim of the study was to compare the results of non-adherent HL-60 cell propagation performed referentially as the batch culture in typical static (i.e. non-agitated) disposable culture flasks (50 cm3 of culture medium) and in ReadyToProcess WAVETM25 bioreactor system (GE Healthcare) equipped with disposable culture bag (300 cm3 of culture medium) subjected to continuous wave-type agitation. The density and viability of HL-60 cells were significantly higher for the bioprocess subjected to wave-type agitation, than in the reference static culture. The values of the specific rate of glucose consumption per cell (rglc=cell) exhibited by HL-60 cells maintained in the system with continuous wave-type agitation was significantly lower (i.e. up to more than 42%) than the values noted for the static culture, for exactly the same time-points of two compared cultures. The results of the studies undoubtedly and comprehensively confirmed the applicability of the studied disposable bioreactor with wave-induced agitation as the right platform for proceeding the propagation of non- adherent HL-60 cells and for providing the culture conditions required by HL-60 cells for sustainable metabolism.}, type={Article}, title={Propagation of non-adherent HL-60 cells in batch cultures maintained in staticand wave-type agitated systems}, URL={http://www.journals.pan.pl/Content/110021/PDF/03_CPE-40-2_07-25.pdf}, doi={10.24425/cpe.2019.126109}, keywords={disposable (single-use) bioreactor, wave-type agitation, non-adherent cell culture, human HL-60 cells}, }