Details

Title

PCR-RFLP method applied for identification of Warmblood Fragile Foal Syndrome carriers in Polish warmblood horses

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2025

Volume

vol. 28

Issue

No 4

Authors

Affiliation

Kamiński, S. : University of Warmia and Mazury, Department of Animal Genetics, Faculty of Animal Bioengineering, Oczapowskiego 5, 10-719 Olsztyn, Poland ; Bejda, J. : University of Warmia and Mazury, Department of Animal Genetics, Faculty of Animal Bioengineering, Oczapowskiego 5, 10-719 Olsztyn, Poland ; Lewczuk, D. : Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Postępu 36A, 05-552 Magdalenka, Poland

Keywords

horse ; genetic defect ; fragile foal ; carrier ; PCR test

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

663–666

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

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Aurich C, Müller-Herbst S, Reineking W, Müller E, Wohlsein P, Gunreben B, Aurich J (2019) Characterization of abortion, stillbirth and non-viable foals homozygous for the Warmblood Fragile Foal Syndrome. Anim Reprod Sci 211: 106202.

Ayad A, Besseboua O, Aissanou S, Stefaniuk-Szmukier M, Piórkowska K, Musiał AD, Długosz B, Kozłowska A, Ropka-Molik K (2022) Profiling of genetic markers useful for breeding decision in Selle Francais horse. J Equine Vet Sci 116:104059

Bellone RR, Ocampo NR, Hughes SS, Le V, Arthur R, Finno CJ, Penedo MC (2020) Warmblood fragile foal syndrome type 1 mutation (PLOD1 c. 2032G> A) is not associated with catastrophic breakdown and has a low allele frequency in the Thoroughbred breed. Equine Vet J 52: 411-414.

Flanagan S, Rowe Á, Duggan V, Markle E, O’Brien M, Barry G (2021) Development of a real-time PCR assay to detect the single nucleotide polymorphism causing Warmblood Fragile Foal Syndrome. PLoS One 16: e0259316. 

Grillos AS, Roach JM, de Mestre AM, Foote AK, Kinglsey NB, Mienaltowski MJ, Bellone RR (2022) First reported case of fragile foal syndrome type 1 in the Thoroughbred caused by PLOD1 c. 2032G> A. Equine Vet J 54: 1086-1093.

Kehlbeck A, Blanco M, Venner M, Freise F, Gunreben B, Sieme H (2025) Warmblood fragile foal syndrome: Pregnancy loss in Warmblood mares. Equine Vet J 57: 915-923. 

Lindgren G, Naboulsi R, Frey R, Solé M (2020) Genetics of skin disease in horses. Vet Clin: Equine Pract 36: 323-339.

Metzger J, Kreft O, Sieme H, Martinsson G, Reineking W, Hewicker-Trautwein M, Distl O (2021) Hanoverian F/W-line contributes to segregation of Warmblood fragile foal syndrome type 1 variant PLOD1:c.2032G>A in Warmblood horses. Equine Vet J 53: 51-59. 

Monthoux C, de Brot S, Jackson M, Bleul U, Walter J (2015) Skin malformations in a neonatal foal tested homozygous positive for Warmblood Fragile Foal Syndrome. BMC Vet Res 11: 12.

Reiter S, Wallner B, Brem G, Haring E, Hoelzle L, Stefaniuk-Szmukier M, Długosz B, Piórkowska K, Ropka-Molik K, Malvick J, Penedo MC, Bellone RR (2020) Distribution of the Warmblood Fragile Foal Syndrome Type 1 Mutation (PLOD1 c.2032G>A) in Different Horse Breeds from Europe and the United States. Genes (Basel) 11: 1518. 

Rowe Á, Flanagan S, Barry G, Katz LM, Lane EA, Duggan V (2021) Warmblood fragile foal syndrome causative single nucleotide polymorphism frequency in horses in Ireland. Ir Vet J 74: 27. 

Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3 – new capabilities and interfaces. Nucleic Acids Res 40: e115.

Wobbe M, Reinhardt F, Reents R, Tetens J, Stock KF (2022) Quantifying the effect of Warmblood Fragile Foal Syndrome on foaling rates in the German riding horse population. PLoS One 17: e0267975. 

 

Date

12.12.2025

Type

Short communication

Identifier

DOI: 10.24425/pjvs.2025.157280
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