Szukana fraza: [Słowa kluczowe = "Pseudomonas"]

Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia

Alexis Nzila Assad Thukair Saravanan Sankara Basheer Chanbasha Musa M. Musa

Archives of Environmental Protection | 2016 | vol. 42 | No 3 | DOI: 10.1515/aep-2016-0028

Słowa kluczowe biodegradation bioremediation Naphthalene Methylobacterium radiotolerans Pseudomonas aeruginosa

Abstrakt

Bioremediation is based on microorganisms able to use pollutants either as a source of carbon or in co-metabolism, and is a promising strategy in cleaning the environment. Using soil contaminated with petroleum products from an industrial area in Saudi Arabia (Jubail), and after enrichment with the polycyclic aromatic hydrocarbon (PAH) naphthalene, a Methylobacterium radiotolerans strain (N7A0) was isolated that can grow in the presence of naphthalene as the sole source of carbon. M. radiotolerans is known to be resistant to gamma radiation, and this is the first documented report of a strain of this bacterium using a PAH as the sole source of carbon. The commonly reported Pseudomonas aeruginosa (strain N7B1) that biodegrades naphthalene was also identified, and gas chromatography analyses have shown that the biodegradation of naphthalene by M. radiotolerans and P. aeruginosa did follow both the salicylate and phthalate pathways.

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Autorzy i Afiliacje

Alexis Nzila

Assad Thukair

Saravanan Sankara

Basheer Chanbasha

Musa M. Musa

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Konrad Chabowski Adam F. Junka Tomasz Piasecki Damian Nowak Karol Nitsch Danuta Smutnicka Marzenna Bartoszewicz Magdalena Moczała Patrycja Szymczyk

Metrology and Measurement Systems | 2017 | vol. 24 | No 2 | 369–380 | DOI: 10.1515/mms-2017-0032

Słowa kluczowe Pseudomonas aeruginosa biofilm interdigitated sensor impedance spectroscopy

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Abstrakt

The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor’s surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.

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Autorzy i Afiliacje

Konrad Chabowski

Adam F. Junka

Tomasz Piasecki

Damian Nowak

Karol Nitsch

Danuta Smutnicka

Marzenna Bartoszewicz

Magdalena Moczała

Patrycja Szymczyk

Wpływ roślin motylkowych i biopreparatów na usunięcie ropopochodnych z gleb o wieloletnim zanieczyszczeniu

Ewa Zabłocka-Godlewska Wioletta Przystaś

Archives of Environmental Protection | 2006 | vol. 32 | No 4 | 53-66

Słowa kluczowe hydrocarbons biodegradation papilionaceous plant bioaugmentation Pseudomonas

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Abstrakt

Zanieczyszczenie substancjami ropopochodnymi stanowi jeden z kluczowych problemów środowiskowych. Jedną z wiciu metod stosowanych w remediacji gruntów jest ich bioaugmentacja. Celem tego procesu jest zwiększenie liczebności i aktywności mikroorganizmów degradujących zanieczyszczenia, czego wynikiem jest przyspieszenie i podniesienie wydajności procesów rozkładu zanieczyszczeń. Innym sposobem podniesienia efektywności procesów biodegradacji jest zastosowanie roślin, szczególnie motylkowych, ze względu na ich zdolności symbiotycznego wiązania azotu atmosferycznego. Celem przeprowadzonych badań było porównanie efektywności zastosowania biopreparatów (komercyjnego i naturalnego) oraz koniczyny białej do remediacji gleb o wieloletnim charakterze skażenia substancjami ropopochodnymi. Badania prowadzone były na glebie pochodzącej z terenu rafinerii w CzechowicachDziedzicach, która została zaklasyfikowana jako silnie zdegradowana (o stosunku C/N = 100/0,7) W trakcie czrcrnastotygodniowych badań prowadzono między innymi analizy mikrobiologiczne (ogólna liczba bakterii, grzybów, promieniowców oraz bakterii z rodzaju Pseudomonasi oraz analizy chemiczne (zawartość frakcji ciężkich, całkowitej zawartości węglowodorów ropopochodnych (TPH) i WWA). Najlepszą modyfikacją okazało się zastosowanie samej koniczyny. Obecność rośI iny wpłynęła na polepszenie warunków wzrostu mikroorganizmów, spowodowała wzrost zawartości azotu i efektywności procesów biodegradacji. W próbie tej po 14 tygodniach badań odnotowano usunięcie 63% TPH, 44% frakcji ciężkich oraz 9% i 80% odpowiednio 4-6- i 2-3-picrścieniowych węglowodorów aromatycznych.

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Autorzy i Afiliacje

Ewa Zabłocka-Godlewska

Wioletta Przystaś

Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight

Samuel Arsène Ntyam Mendo Dorice Nguelo Dzumafo Laure Brigitte Kouitcheu Mabeku Severin Tchameni Nguemezi Lambert Sameza Modeste Rosalie Anne Ngono Ngane

Journal of Plant Protection Research | 2023 | vol. 63 | No 3 | 350–365 | DOI: 10.24425/jppr.2023.146875

Słowa kluczowe biocontrol fluorescent Pseudomonas Phytophthora colocasiae rhizobacteria taro

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Abstrakt

Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future.

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Autorzy i Afiliacje

Samuel Arsène Ntyam Mendo

1

e-mail:

ORCID:

Dorice Nguelo Dzumafo

2

Laure Brigitte Kouitcheu Mabeku

3

Severin Tchameni Nguemezi

2

e-mail:

ORCID:

Lambert Sameza Modeste

2

Rosalie Anne Ngono Ngane

2

Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon
Department of Biochemistry, Faculty of Sciences, University of Douala, Douala, Cameroon
Department of Microbiology, Faculty of Science, University of Yaoundé 1, Yaounde, Cameroon

Diversity of Pseudomonas species associated with soft rot of plants in Poland

Lena Żołbowska Henryk Pospieszny

Journal of Plant Protection Research | 2001 | No 1 | 67-76

Słowa kluczowe Pseudomonas sp. PCR heterogeneity identification occurrence

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Abstrakt

A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.

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Autorzy i Afiliacje

Lena Żołbowska

Henryk Pospieszny

Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

Deori Monali Jayamohan Nellickal Subramanyan Kumudini Belur Satyan

Journal of Plant Protection Research | 2018 | vol. 58 | No 1 | DOI: 10.24425/119116

Słowa kluczowe siderophore hydroxamate fluorescent Pseudomonas succinate medium iron-binding mutation

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Abstrakt

Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.

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Autorzy i Afiliacje

Deori Monali

Jayamohan Nellickal Subramanyan

Kumudini Belur Satyan

Hemorrhagic pneumonia in neonatal minks in Greece concomitant with Leismania infantum detection

G. Filioussis E. Petridou D. Papadopoulos E. Karavanis E. Morgan C. Billinis E. Papadopoulos

Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 2 | 405-408 | DOI: 10.24425/122608

Słowa kluczowe mink hemorrhagic pneumonia Pseudomonas aeruginosa Leismania infantum Greece

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Abstrakt

In the present study a severe outbreak of hemorrhagic pneumonia (HP) in neonatal minks concomitant with Leismania infantum (L. infantum) detection is reported. The outbreak took place on a Greek mink farm and affected 1,362 mink kits, with 524 dying. Macroscopic lesions of 14 necropsied affected kits were confined to the respiratory system with dark red, consolidated lung lobes and to the small intestine with severe, acute, hemorrhagic and necrotic enteritis. Microscopic examination of lung sections revealed severe hemorrhagic pyogranulomatous pneumonia. Bacteria were obtained in pure culture from the lungs of all necropsied animals and were confirmed as Pseudomonas aeruginosa (P. aeruginosa). Three out of 14 (21.4%) animals were positive for the presence of L. infantum DNA. The outbreak was attributed to the infection of minks with P. aeruginosa, possibly as a consequence of being immuno-suppressed by L. infantum. Further research is necessary, especially on the pathogenesis of P. aeruginosa/L. infantum co-infection and the implications of this interaction on HP disease outcome.

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Autorzy i Afiliacje

G. Filioussis

E. Petridou

D. Papadopoulos

E. Karavanis

E. Morgan

C. Billinis

E. Papadopoulos

Effects of inoculation with mycorrhizae and the benefits of bacteria on physicochemical and microbiological properties of soil, growth, productivity and quality of table grapes grown under Mediterranean climate conditions

Salah Ed-dine Samri Kamal Aberkani Mourad Said Khadija Haboubi Hassan Ghazal

Journal of Plant Protection Research | 2021 | vol. 61 | No 4 | 337-346 | DOI: 10.24425/jppr.2021.139242

Słowa kluczowe Glomus iranicum fertilizer nutrition Pseudomonas putida symbiosis yield

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Abstrakt

Excessive use of chemical fertilizers, in agriculture, has negative impacts on water, soil and affects the environment and health. In recent decades, researchers have been interested in the natural benefits of natural microorganisms and how they could be a good alternative to the use of chemical fertilizers. The aim of this study was to investigate the effect of soil inoculation with strains of mycorrhizae and beneficial bacteria on soil properties and productivity of table grapes. Field trials were conducted on a commercial table grape production farm ( Vitis vinifiera cv. Mousca), located in northeastern Morocco. Twelve-yearold plants were used. Control plants were not inoculated (T1). The prototype plants were inoculated with 1.2 × 104 of Glomus iranicum var. tenuihypharum/100 g (T2), a mixture of 1/2 concentration of Glomus iranicum var. tenuihypharum and 1/2 concentration of Pseudomonas putida (T3) and 1 × 108 CFU ∙ g–1 of Pseudomonas putida (T4). The inoculations were realized twice; the first inoculation was completed on July 19, 2019 while the second inoculation on February 21, 2020. Soil analyses were carried out, both physicochemical (pH, electrical conductivity (EC), salinity, % of dry matter) and microbiological properties (total flora, fungi and actinobacteria). Plant growth (length of the plant, number and diameter of sticks, number of clusters per tree, number of nodes per stick, distance between nodes and bud burst), yield and fruit quality (number of berries per cluster, cluster weight, cluster length and width, pH, Brix degrees, acidity, EC and % dry matter) were measured. Results showed slight trends regarding the effects of treatments on the physicochemical and microbiological properties of the soil, plant growth and fruit quality. The number of clusters was significantly higher in Glomus (T2) Pseudomonas (T4) and Glomus than in control treatments.

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Autorzy i Afiliacje

Salah Ed-dine Samri

1

e-mail:

ORCID:

Kamal Aberkani

1

e-mail:

ORCID:

Mourad Said

1

Khadija Haboubi

2

e-mail:

ORCID:

Hassan Ghazal

3

e-mail:

ORCID:

Biology and Geology, Plolydisciplinary Faculty of Nador, University Mohammed Fisrt, Selonane, Morocco
Environment, National School of Applied Sciences, University Abdelmalek Essaadi, Al Hoceima, Morocco
Bioinformatics, National Center for Scientific and Technical Research, Rabat, Morocco

Bacterial selection of the Pseudomonas genus with the capacity to treat water and contaminated soils

Henry Joel Escudero-López Karla Anabel Serrano-Carrillo Carlos Rodrigo Jácome-Pilco Herminia del Rosario Sanaguano-Salguero Isidro Favian Bayas-Morejón

Journal of Water and Land Development | 2022 | No 53 | 238-241 | DOI: 10.24425/jwld.2022.140803

Słowa kluczowe bioremediation characterisation contaminated water contaminated soils Ecuadorian Amazon Pseudomonas genus

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Abstrakt

In the present work, bacteria of the Pseudomonas genus native to the Ecuadorian Amazon with the capacity to treat contaminated water and soils were selected. For this purpose, 20 soil samples from Amazon region with evidence of contamination were analysed. For identification, each sample was assigned a code according to the sampling area: Joya de los Sachas (S), Minga (M) and Siete de Julio-Shushufindi (SH). The cultures were performed in the combination of Bushnell Hass (BH) + Luria Bertani (LB) and Müeller-Hinton (MH) + Brucella agar (BA) media, all with the addition of diesel to verify their efficacy in the growth of bacteria capable of surviving in contaminated media. The combination with ideal results was that of BH + LB, by means of Gram-staining it was determined that 19 of the samples had interest microorganisms. To characterize the isolates at the species level, biochemical tests of: catalase, citrate, glucose, hemolytic activity and urease were applied, which allowed to confirm the existence of the Pseudomonas of interest. The results indicated that P. stutzeri (in samples S1 and M1), P. aeruginosa (in SH2 and SH5) and P. putida (in S7, S8, S10 and SH4) obtaining a total of 8 isolates (40%) of interest from the initial 19. With the results obtained from this work, an optimal culture method was standardized for the selection of bacteria with potential for treating contaminated soils and water.

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Autorzy i Afiliacje

Henry Joel Escudero-López

1

e-mail:

ORCID:

Karla Anabel Serrano-Carrillo

2

e-mail:

ORCID:

Carlos Rodrigo Jácome-Pilco

1

e-mail:

ORCID:

Herminia del Rosario Sanaguano-Salguero

1

e-mail:

ORCID:

Isidro Favian Bayas-Morejón

1

e-mail:

ORCID:

State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador
San Pedro Educational Unit, Department of Education, Guaranda, Ecuador

Carbonic anhydrase production by Pseudomonas fragi

Andrzej Tietz Małgorzata M. Jaworska

Chemical and Process Engineering: New Frontiers | 2023 | vol. 44 | No 1 | e6 | DOI: 10.24425/cpe.2023.144692

Słowa kluczowe carbonic anhydrase Pseudomonas fragi calcium carbonate phase of growth

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Abstrakt

Carbonic anhydrase is an important enzyme that can play a significant role in the processes of lowering carbon dioxide concentration in the atmosphere. The aim of the work was to investigate the extracellular carbonic anhydrase (CA) production by the bacteria Pseudomonas fragi. In the research, we focused on the evaluation of the phase of bacterial growth correlated with carbonic anhydrase production and on the evaluation of induction of CA production by calcium carbonate concentration in the nutrient medium. Presented data indicated that calcium carbonate can serve as the only carbon source for Pseudomonas fragi, inducing carbonic anhydrase secretion to culture broth. The enzyme was produced mainly in the adaptation growth phase reaching the maximal activity at the end of this phase or at the beginning of the growth phase. The maximal enzyme activity detected in all batches was at a similar level. The enzyme activity was constant but lower in the exponential phase growth. Therefore, the enzyme production is not growth-dependent, but it is correlated with bacteria adaptation to cultivation conditions.

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Andrzej Tietz

1

Małgorzata M. Jaworska

1

e-mail:

ORCID:

Warsaw University of Technology, Faulty of Chemical and Process Engineering, ul. Warynskiego 1, 00-645 Warsaw, Poland

Outbreak of Pseudomonas aeruginosa mastitis in a dairy cow herd in northern Greece and its control with an autogenous vaccine

E.J. Petridou I.A. Fragkou S.Q. Lafi N.D. Giadinis

Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 2 | 303-305 | DOI: 10.24425/pjvs.2021.137666

Słowa kluczowe dairy cows Pseudomonas aeruginosa clinical mastitis control autogenous vaccine

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Abstrakt

In a dairy cow herd consisted of herd of 200 lactating Holstein-Friesian cows and heifers, clinical signs of mastitis in 40 out of 170 animals were observed. Treatments with antibiotics were proved ineffective. Milk bacterial cultures from 15 affected animals revealed Pseudomonas aeruginosa. An autogenous vaccine was administered subcutaneously, twice in a month period, to all adults. Cases of clinical mastitis declined significantly (p<0.0001) during next 3 months.

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Autorzy i Afiliacje

E.J. Petridou

1

I.A. Fragkou

2

S.Q. Lafi

3

N.D. Giadinis

4

Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
Veterinary Service, Ministry of Rural Development and Food, Athens, Greece
Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan
Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece

Influence of Fusarium oxysporum f. sp. Cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol production by Pseudomonas fluorescens Migula in banana rhizosphere

Thangavelu Saravanan Mari Muthusamy

Journal of Plant Protection Research | 2006 | vol. 46 | No 3 | 241-253

Słowa kluczowe 2,4-diacetylpholoroglucinol Pseudomonas fluorescens banana Fusarium oxysporum f. sp. cubense

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Abstrakt

Influence of Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol (DAPG) production in the rhizosphere of banana cultivar Rasthali by Pseudomonas fluorescens was investigated. The purified extracts of Pfm strain of P. fluorescens isolated from banana rhizosphere inhibited the growth and spore germination of F. oxysporum f. sp. cubense under laboratory conditions. DAPG extracted from the cultures of the strain was observed as distinct spots in thin layer chromatographic plates at Rf value of 0.88. The extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense eluted at retention time ranges from 20.00 min to 21.30 min. The quantity of DPAG production was less in the extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense as compared to P. fluorescens alone inoculated soil. The talc formulation of Pfm strain also reduced vascular discolouration due to the pathogen in banana plants when inoculated at 15 g/plant.

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Thangavelu Saravanan

Mari Muthusamy

Escherichia coli a wybrane grupy bakterii oportunistycznie patogennych i patogennych w wodzie kąpielisk jeziora Wigry

Ewa Korzeniewska

Archives of Environmental Protection | 2005 | vol. 31 | No 2 | 69-84

Słowa kluczowe Escherichia coli Staphylococcus aureus Aeromonas hydrophila Pseudomonas aeruginosa bathing sites lake

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Abstrakt

W sezonie letnim (od czerwca do września) 1995-1999 prowadzono badania składu ilościowego i jakościowego bakterii z rodziny Enterobacteriaceae (w tym Escherichia coli i Salmonella sp.) oraz bakterii potencjalnie chorobotwórczych Aeromonas hydrophila, Pseudomonas aeruginosa i Staphylococcus sp. w wodzie 8 wybranych kąpielisk jeziora Wigry. Bakterie Aeromonas hydrophila występowały w wodzie wszystkich kąpielisk, najliczniej zaś w wodzie pobieranej z miejsc o zwiększonej trofii. Bakterie Pseudomonas aeruginosa izolowano rzadko, niezależnie od czasu i miejsca poboru prób. Bakterie z rodzaju Salmonella, gatunku Escherichia coli i Staphylococcus aureus stwierdzano odpowiednio w 37 (22,6%), 68 (42,4%) i 90 (55%) na ogólną liczbę l 60 przebadanych prób wody kąpielisk jeziora Wigry. Stwierdzane w wodzie badanych kąpielisk (zwłaszcza w miejscowościach Stary Folwark, Leszczewek, i Rosochaty Róg) bakterie Salmonella sp., Staphylococcus aureus i Aeromonas hydrophila (nawet przy braku bakterii z gatunku Escherichia coli) sugerują, iż ocena przydatności wód jeziora Wigry dla celów kąpielowych na podstawie liczebności bakterii grupy coli typu kałowego może nie odzwierciedlać w pełni jej bezpieczeństwa dla kąpiących się. Wyniki przeprowadzonych badań sugerują konieczność uwzględnienia w ocenie przydatności wód powierzchniowych jeziora Wigry dla celów rekreacji (zwłaszcza kąpieli) badań na obecność Salmonella sp., Staphylococcus aureus, Aeromonas hydrophi/a i Pseudomonas aeruginosa. Ostatnie 3 gatunki niezwiązane bezpośrednio z zanieczyszczeniami kałowymi bywają, bowiem przyczyną różnorodnych schorzeń skórnych, jamy nosowogardzielowej, oczu, ucha środkowego i szeregu innych spotykanych wśród kąpiących się w zanieczyszczonej wodzie.

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Ewa Korzeniewska

In vitro antibacterial effects of non-thermal atmospheric plasma irradiation on Staphylococcus pseudintermedius and Pseudomonas aeruginosa

S. Bae D. Lim D. Kim J. Jeon T. Oh

Polish Journal of Veterinary Sciences | 2020 | vol. 23 | No 1 | 13-19 | DOI: 10.24425/pjvs.2019.131414

Słowa kluczowe antibacterial effects cold-atmospheric plasma dog Staphylococcus pseudinter- medius Pseudomonas aeruginosa

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Abstrakt

In the last decade, atmospheric plasma has been used to treating bacterial infections in humans due to its bactericidal effects; however, its efficacy in dogs is unclear. This study evaluated the in vitro bactericidal efficacy of atmospheric plasma on Staphylococcus pseudinter- medius and Pseudomonas aeruginosa, two of the most important bacterial agents isolated from canine pyodermas. Three isolates each of S. pseudintermedius and P. aeruginosa obtained from dogs with pyoderma were subjected to atmospheric plasma. The isolates from the control group were not exposed to plasma, while those from the treatment groups were exposed to plasma for 15 (7.5 J/cm2), 30 (15 J/cm2), 60 (30 J/cm2), or 90 (45 J/cm2) seconds. After each treatment, a reduction in colony formation was observed. Bacterial viability was evaluated using the LIVE/ DEAD® BacLight™ Bacterial Viability Kit. The antibacterial effects were evaluated with Image J software and significance was assessed statistically in comparison to the control group. The bactericidal effect of atmospheric plasma against both bacteria increased significantly in a time-dependent manner. These results demonstrate the bactericidal capacity of atmospheric plasma, and suggest that it could serve as an alternative treatment method for canine pyoderma. Further studies are needed to evaluate the safety and efficacy of atmospheric plasma in dogs.

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S. Bae

D. Lim

D. Kim

J. Jeon

T. Oh

Antagonistic bacteria and fungi limiting potato infection by soil-borne pathogenic fungi

Danuta Pięta Elżbieta Patkowska

Journal of Plant Protection Research | 2003 | vol. 43 | No 2 | 97-104

Słowa kluczowe potato phytopathogens Bacillus spp. Pseudomonas spp. Trichodermaspp. Gliocladium spp. Penicillium spp.

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The purpose of the presented studies was to determine the species composition of the fungi occurring on the underground parts of potato at an thesis and to establish the qualitative and quantitative composition ofmicroorganisms living in the soil environment of this plant. Besides, the studies aimed at finding antagonistic microorganisms inhibiting potato infection by soil-borne phytopathogens. The mycological analysis showed that the symptoms of necrosis on the roots and the stem base of potato were caused by Alternaria alternata, Fusarium spp., Colletotrichum coccodes and Rhizoctonia so/ani. The microbiological analysis of the potato rhizosphere gave twice as many bacteria and fungi as from the non-rhizosphere soil. The dominating pathogenic fungi in the examined soil samples were Fusarium spp., A. alternata and R. so/ani. Three times as many antagonistic bacteria Pseudomonas spp. and Bacillus spp. and more than twice as many antagonistic fungi (Penicillium spp., Trichoderma spp., Gliocladium spp.) were obtained from the potato rhizosphere as compared to the non-rhizosphere soil.

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Danuta Pięta

Elżbieta Patkowska

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